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rdf:type
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Description
| - Background: The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results: We analyzed the DNA-damage response after ultraviolet A (UVA) and. irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1 beta (HP1 beta) level. Moreover, Forster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1 beta that was dependent on a functional chromo shadow domain of HP1 beta. Thus, overexpression of HP1 beta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.
- Background: The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results: We analyzed the DNA-damage response after ultraviolet A (UVA) and. irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1 beta (HP1 beta) level. Moreover, Forster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1 beta that was dependent on a functional chromo shadow domain of HP1 beta. Thus, overexpression of HP1 beta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin. (en)
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Title
| - HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
- HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs (en)
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skos:prefLabel
| - HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
- HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs (en)
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skos:notation
| - RIV/68081707:_____/14:00441300!RIV15-GA0-68081707
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - I, P(GA13-07822S), P(GAP302/10/1022), P(GBP302/12/G157)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/14:00441300
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - DNA-damage response; DNA repair; Irradiation (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Bártová, Eva
- Fulneček, Jaroslav
- Kovařík, Aleš
- Legartová, Soňa
- Stixová, Lenka
- Matula, Pa.
- Suchánková, Jana
- Raška, I.
- Hrušková, Tereza
- Sehnalová, Petra
- Sorokin, D. V.
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
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