About: Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 %22Rods and Rings%22 Inclusions

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About: Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 %22Rods and Rings%22 Inclusions Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Inosine-5-monophosphate dehydrogenase catalyzes the critical step in the de novo synthesis of guanosine nucleotides: the oxidation of inosine monophosphate to xanthosine monophosphate. This reaction can be inhibited by specific inhibitors, such as ribavirin or mycophenolic acid, which are widely used in clinical treatment when required to inhibit the proliferation of viruses or cells. However, it was recently found that such an inhibition affects the cells, leading to a redistribution of IMPDH2 and the appearance of IMPDH2 inclusions in the cytoplasm. According to their shape, these inclusions have been termed Rods and Rings (R&R). In this work, we focused on the subcellular localization of IMPDH2 protein and the ultrastructure of R&R inclusions. Using microscopy and western blot analysis, we show the presence of nuclear IMPDH2 in human cells. We also show that the nuclear pool has an ability to form Rod structures after inhibition by ribavirin. Concerning the ultrastructure, we observed that R&R inclusions in cellulo correspond to the accumulation of fibrous material that is not surrounded by a biological membrane. Inosine-5-monophosphate dehydrogenase catalyzes the critical step in the de novo synthesis of guanosine nucleotides: the oxidation of inosine monophosphate to xanthosine monophosphate. This reaction can be inhibited by specific inhibitors, such as ribavirin or mycophenolic acid, which are widely used in clinical treatment when required to inhibit the proliferation of viruses or cells. However, it was recently found that such an inhibition affects the cells, leading to a redistribution of IMPDH2 and the appearance of IMPDH2 inclusions in the cytoplasm. According to their shape, these inclusions have been termed Rods and Rings (R&R). In this work, we focused on the subcellular localization of IMPDH2 protein and the ultrastructure of R&R inclusions. Using microscopy and western blot analysis, we show the presence of nuclear IMPDH2 in human cells. We also show that the nuclear pool has an ability to form Rod structures after inhibition by ribavirin. Concerning the ultrastructure, we observed that R&R inclusions in cellulo correspond to the accumulation of fibrous material that is not surrounded by a biological membrane. (en)
Title	Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 %22Rods and Rings%22 Inclusions Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 %22Rods and Rings%22 Inclusions (en)
skos:prefLabel	Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 %22Rods and Rings%22 Inclusions Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 %22Rods and Rings%22 Inclusions (en)
skos:notation	RIV/68081707:_____/14:00440593!RIV15-GA0-68081707
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(EE2.3.30.0030), P(GBP302/12/G157)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Bártová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	51808
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/14:00440593
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Inosine-5-monophosphate dehydrogenase; inhibitors of IMPDH; Rods and Rings (en)
http://linked.open.../riv/klicoveSlovo	Inosine-5-monophosphate dehydrogenase Rods and Rings inhibitors of IMPDH
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1C75016E0BD9]
http://linked.open...i/riv/nazevZdroje	Journal of Histochemistry & Cytochemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions Development of human resources in the field of cell biology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	62
http://linked.open...iv/tvurceVysledku	Bártová, Eva Kováčik, L. Raška, I. Šmigová, J. Juda, P.
http://linked.open...ain/vavai/riv/wos	000342836200004
issn	0722-186X
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1369/0022155414543853

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