About: Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface

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About: Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Electrochemical biosensors have the unique ability to convert biological events directly into electrical signals suitable for parallel analysis. Here we utilize specific properties of constant current chronopotentiometric stripping (CPS) in the analysis of protein and DNA-protein complex nanolayers. Rapid potential changes at high negative current intensities (I-str) in CPS are utilized in the analysis of DNA-protein interactions at thiol-modified mercury electrodes. P53 core domain (p53CD) sequence-specific binding to DNA results in a striking decrease in the electrocatalytic signal of free p53. This decrease is related to changes in the accessibility of the electroactive amino acid residues in the p53CD-DNA complex. By adjusting I-str and temperature, weaker non-specific binding can be eliminated or distinguished from the sequence-specific binding. The method also reflects differences in the stabilities of different sequence-specific complexes, including those containing spacers between half-sites of the DNA consensus sequence. Electrochemical biosensors have the unique ability to convert biological events directly into electrical signals suitable for parallel analysis. Here we utilize specific properties of constant current chronopotentiometric stripping (CPS) in the analysis of protein and DNA-protein complex nanolayers. Rapid potential changes at high negative current intensities (I-str) in CPS are utilized in the analysis of DNA-protein interactions at thiol-modified mercury electrodes. P53 core domain (p53CD) sequence-specific binding to DNA results in a striking decrease in the electrocatalytic signal of free p53. This decrease is related to changes in the accessibility of the electroactive amino acid residues in the p53CD-DNA complex. By adjusting I-str and temperature, weaker non-specific binding can be eliminated or distinguished from the sequence-specific binding. The method also reflects differences in the stabilities of different sequence-specific complexes, including those containing spacers between half-sites of the DNA consensus sequence. (en)
Title	Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface (en)
skos:prefLabel	Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface (en)
skos:notation	RIV/68081707:_____/14:00435478!RIV15-GA0-68081707
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-00956S), P(GA13-36108S), P(GAP301/11/2055), P(GBP305/12/G034)
http://linked.open...iv/cisloPeriodika	MAY2014
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Navrátilová, Lucie Paleček, Emil Ostatná, Veronika Černocká, Hana Brázdová, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	14117
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/14:00435478
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Deoxyribonucleic acid-protein binding; Tumor suppressor protein p53; Electrochemical sensing (en)
http://linked.open.../riv/klicoveSlovo	Deoxyribonucleic acid-protein binding Electrochemical sensing Tumor suppressor protein p53
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[2DD39D433992]
http://linked.open...i/riv/nazevZdroje	Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for RNA Biology Emerging roles for the proteins of Anterior Gradient family in cancer development New methods of analysis of proteins and their glycosylation in cancer - combination of electrochemistry, microfludic biosensors and mass spectrometry. Interakce proteinů rodiny p53 s biologicky významnými kvadruplexy DNA
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	828
http://linked.open...iv/tvurceVysledku	Brázdová, Marie Navrátilová, Lucie Ostatná, Veronika Paleček, Emil Černocká, Hana
http://linked.open...ain/vavai/riv/wos	000336336900001
issn	0003-2670
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.aca.2014.03.029

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