About: How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles?

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Background: DNA methylation plays a key role in development, contributes to genome stability, and may also respond to external factors supporting adaptation and evolution. To connect different types of stimuli with particular biological processes, identifying genome regions with altered 5-methylcytosine distribution at a genome-wide scale is important. Many researchers are using the simple, reliable, and relatively inexpensive Methylation Sensitive Amplified Polymorphism (MSAP) method that is particularly useful in studies of epigenetic variation. However, electrophoretic patterns produced by the method are rather difficult to interpret, particularly when MspI and HpaII isoschizomers are used because these enzymes are methylation-sensitive, and any C within the CCGG recognition motif can be methylated in plant DNA. Background: DNA methylation plays a key role in development, contributes to genome stability, and may also respond to external factors supporting adaptation and evolution. To connect different types of stimuli with particular biological processes, identifying genome regions with altered 5-methylcytosine distribution at a genome-wide scale is important. Many researchers are using the simple, reliable, and relatively inexpensive Methylation Sensitive Amplified Polymorphism (MSAP) method that is particularly useful in studies of epigenetic variation. However, electrophoretic patterns produced by the method are rather difficult to interpret, particularly when MspI and HpaII isoschizomers are used because these enzymes are methylation-sensitive, and any C within the CCGG recognition motif can be methylated in plant DNA. (en)
Title	How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles? How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles? (en)
skos:prefLabel	How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles? How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles? (en)
skos:notation	RIV/68081707:_____/14:00428114!RIV15-GA0-68081707
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-10057S), P(GA206/09/1751), P(GBP501/12/G090)
http://linked.open...iv/cisloPeriodika	JAN 2014
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Fulneček, Jaroslav Kovařík, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	19810
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/14:00428114
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MSAP; DNA methylation; Methylcytosine (en)
http://linked.open.../riv/klicoveSlovo	MSAP DNA methylation Methylcytosine
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[501EC02B45BB]
http://linked.open...i/riv/nazevZdroje	B M C Genetics
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Struktura, exprese a evoluce genů pro ribosomální RNA u rostlin: novými přistupy k řešení starých otázek The impact of genomic shock associated with interspecific hybridization and polyploidization on evolution of rDNA loci in young invasive weeds. Evolution and Function of Complex Plant Genomes
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Fulneček, Jaroslav Kovařík, Aleš
http://linked.open...ain/vavai/riv/wos	000329970400001
issn	1471-2156
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1186/1471-2156-15-2

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