About: Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture

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About: Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In plants, silencing is usually accompanied by DNA methylation and heterochromatic histone marks. We studied these epigenetic modifications in different epialleles of 35S promoter (P35S)-driven tobacco transgenes. In locus 1, the T-DNA was organized as an inverted repeat, and the residing neomycin phosphotransferase II reporter gene (P35S-nptII) was silenced at the posttranscriptional (PTGS) level. Transcriptionally silenced (TGS) epialleles were generated by trans-acting RNA signals in hybrids or in a callus culture. PTGS to TGS conversion in callus culture was accompanied by loss of the euchromatic H3K4me3 mark in the transcribed region of locus 1, but this change was not transmitted to the regenerated plants from these calli. In contrast, cytosine methylation that spread from the transcribed region into the promoter was maintained in regenerants. Also, the TGS epialleles generated by trans-acting siRNAs did not change their active histone modifications. In plants, silencing is usually accompanied by DNA methylation and heterochromatic histone marks. We studied these epigenetic modifications in different epialleles of 35S promoter (P35S)-driven tobacco transgenes. In locus 1, the T-DNA was organized as an inverted repeat, and the residing neomycin phosphotransferase II reporter gene (P35S-nptII) was silenced at the posttranscriptional (PTGS) level. Transcriptionally silenced (TGS) epialleles were generated by trans-acting RNA signals in hybrids or in a callus culture. PTGS to TGS conversion in callus culture was accompanied by loss of the euchromatic H3K4me3 mark in the transcribed region of locus 1, but this change was not transmitted to the regenerated plants from these calli. In contrast, cytosine methylation that spread from the transcribed region into the promoter was maintained in regenerants. Also, the TGS epialleles generated by trans-acting siRNAs did not change their active histone modifications. (en)
Title	Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture (en)
skos:prefLabel	Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture (en)
skos:notation	RIV/68081707:_____/13:00422432!RIV14-GA0-68081707
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-10057S), P(GBP501/12/G090), P(GPP501/11/P667)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kovařík, Aleš Křížová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	73104
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/13:00422432
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	JMJC DOMAIN PROTEIN; DNA METHYLATION; CYTOSINE METHYLATION (en)
http://linked.open.../riv/klicoveSlovo	CYTOSINE METHYLATION JMJC DOMAIN PROTEIN DNA METHYLATION
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[0B60B11B46E8]
http://linked.open...i/riv/nazevZdroje	Epigenetics
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Struktura, exprese a evoluce genů pro ribosomální RNA u rostlin: novými přistupy k řešení starých otázek Reprogramování epigenetického stavu transgenních a endogenních lokusů v průběhu dediferenciace a regenerace rostlin. Evolution and Function of Complex Plant Genomes
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Křížová, Kateřina Kovařík, Aleš Depicker, A.
http://linked.open...ain/vavai/riv/wos	000327623100011
issn	1559-2294
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.4161/epi.24613

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