About: Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of our work was to evaluate mechanisms leading to radiosensitization of MOLT-4 leukemia cells following valproic acid (VA) treatment. Cells were pretreated with 2 mM VA for 24 h followed by irradiation with a dose of 0.5 or 1 Gy. The effect of both noxae, alone and combined, was detected 1 and 24 hours after the irradiation. Induction of apoptosis was evaluated by a flow cytometry. The extent of DNA damage was further determined by phosphorylation of histone H2AX using confocal microscopy. Changes in protein expression were identified by SDS-PAGE/immunoblotting. Two-millimolar VA increased apoptosis induction after irradiation as well as phosphorylation of H2AX and provokes an increase in the level of p53 and its phosphorylation at Ser392 in 4 h post-irradiation. Likewise, p21 protein reached its maximal expression in 4 h after the irradiation of VA-treated cells. The aim of our work was to evaluate mechanisms leading to radiosensitization of MOLT-4 leukemia cells following valproic acid (VA) treatment. Cells were pretreated with 2 mM VA for 24 h followed by irradiation with a dose of 0.5 or 1 Gy. The effect of both noxae, alone and combined, was detected 1 and 24 hours after the irradiation. Induction of apoptosis was evaluated by a flow cytometry. The extent of DNA damage was further determined by phosphorylation of histone H2AX using confocal microscopy. Changes in protein expression were identified by SDS-PAGE/immunoblotting. Two-millimolar VA increased apoptosis induction after irradiation as well as phosphorylation of H2AX and provokes an increase in the level of p53 and its phosphorylation at Ser392 in 4 h post-irradiation. Likewise, p21 protein reached its maximal expression in 4 h after the irradiation of VA-treated cells. (en)
Title	Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells (en)
skos:prefLabel	Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells (en)
skos:notation	RIV/68081707:_____/12:00389863!RIV13-AV0-68081707
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Lukášová, Emilie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	176918
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/12:00389863
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	HISTONE DEACETYLASE INHIBITOR; ENHANCES RADIATION SENSITIVITY; TRANSFORMED-CELLS (en)
http://linked.open.../riv/klicoveSlovo	ENHANCES RADIATION SENSITIVITY HISTONE DEACETYLASE INHIBITOR TRANSFORMED-CELLS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[69AFF8203FD0]
http://linked.open...i/riv/nazevZdroje	Molecular Biology
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	46
http://linked.open...iv/tvurceVysledku	Lukášová, Emilie Řezáčová, M. Vávrová, J. Knížek, J. Tichý, A. Köhlerová, R. Mazánková, N. Muthna, D.
http://linked.open...ain/vavai/riv/wos	000302960100013
issn	0026-8933
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1134/S0026893312010141

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