About: Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications

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About: Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Modified nucleoside triphosphates bearing [Ru (bpy)3]2+ and [Os(bpy)3]2+ complexes attached via acetylene linker were prepared by cross-coupling reactions and were further used for incorporation to DNA by polymerase. The metal complexes were used as luminescent or redox label for detection of DNA. Multi-color redox labeling of DNA by four different labels was also developed. Modified nucleoside triphosphates bearing [Ru (bpy)3]2+ and [Os(bpy)3]2+ complexes attached via acetylene linker were prepared by cross-coupling reactions and were further used for incorporation to DNA by polymerase. The metal complexes were used as luminescent or redox label for detection of DNA. Multi-color redox labeling of DNA by four different labels was also developed. (en) Modifikované nukleosid trifosfáty nesoucí [Ru (bpy)3]2+ a [Os(bpy)3]2+ komplexy připojené přes acetylenový můstek byly připraveny cross-coupling reakcemi a byly dále použity pro inkorporace do DNA katalyzované polymerasou. Komplexy kovů byly potom využity jako luminiscenční nebo redoxní značky pro detekci DNA. Vícebarevné kódování DNA bylo rovněž vyvinuto s použitím 4 různých redoxních značek. (cs)
Title	Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications DNA obsahující modifikované báze označené [Ru (bpy)3]2+ a [Os(bpy)3]2+ komplexy: konstrukce inkorporacemi modifikovaných nukleosid trofosfátů polymerasou, elektrochemické a luminiscenční vlastnosti a aplikace (cs) Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications (en)
skos:prefLabel	Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications DNA obsahující modifikované báze označené [Ru (bpy)3]2+ a [Os(bpy)3]2+ komplexy: konstrukce inkorporacemi modifikovaných nukleosid trofosfátů polymerasou, elektrochemické a luminiscenční vlastnosti a aplikace (cs) Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications (en)
skos:notation	RIV/68081707:_____/09:00324504!RIV09-AV0-68081707
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/07/1195), P(IAA400040901), P(LC06035), P(LC512), Z(AV0Z40550506), Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Černocká, Hana Šebest, Peter Havran, Luděk Fojta, Miroslav Horáková, Petra Pivoňková, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	304721
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/09:00324504
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nucleotides; polymerase; DNA; metal complexes (en)
http://linked.open.../riv/klicoveSlovo	polymerase DNA metal complexes nucleotides
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[D869DA34F17C]
http://linked.open...i/riv/nazevZdroje	Chemistry - A European Journal
http://linked.open...in/vavai/riv/obor	CC
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine. Center for biomolecules and complex molecular systems Analysis of DNA structure and interactions using electrochemical techniques and chemical probes. Novel techniques and sensors for DNA damage detection DNA labeling with redox markers for electrochemical sensing. Applications in analysis of nucleotide sequences and molecular diagnostics
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Fojta, Miroslav Havran, Luděk Hocek, Michal Horáková, Petra Pohl, Radek Černocká, Hana Pivoňková, Hana Kalachová, Lubica Cahová, Hana Vrábel, Milan Šebest, Peter
http://linked.open...ain/vavai/riv/wos	000263093700012
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Biophysics of dynamic structures and functions of biological systems
issn	0947-6539
number of pages	11 (xsd:int)

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