About: Native, denatured and reduced BSA enhancement of chronopotentiometric peak H by guanidinium chloride

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About: Native, denatured and reduced BSA enhancement of chronopotentiometric peak H by guanidinium chloride Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	It was shown that using constant current chronopotentiometric stripping peak H, minute amounts of denatured and reduced bovine serum albumin (BSA) can be easily discriminated from native BSA. Peak H, which is due to catalytic hydrogen evolution, is greatly enhanced in the presence of non-denaturing concentrations of guanidinium chloride. The course of BSA reduction and denaturation can be followed and traces of the damaged protein can be detected in native BSA samples. It was shown that using constant current chronopotentiometric stripping peak H, minute amounts of denatured and reduced bovine serum albumin (BSA) can be easily discriminated from native BSA. Peak H, which is due to catalytic hydrogen evolution, is greatly enhanced in the presence of non-denaturing concentrations of guanidinium chloride. The course of BSA reduction and denaturation can be followed and traces of the damaged protein can be detected in native BSA samples. (en) Bylo zjištěno, že pomocí píku H při chronopotenciometrii konstantním proudem minimální množství denaturovaného a redukovaného hovězího serumalbuminu (BSA) mohou být snadno odlišena od nativního BSA. Pík H, za který je odpovědné katalytické vylučování vodíku, je silně zvýšen v přítomnost nedenaturačních koncentrací guanidinium chloridu. Průběh redukce a denaturace BSA lze sledovat a stopy poškozeného proteinu mohou být detekovány ve vzorcích nativního BSA. (cs)
Title	Native, denatured and reduced BSA enhancement of chronopotentiometric peak H by guanidinium chloride Nativní, denaturovaný a redukovaný BSA. Zvýraznění chronopotenciometrického píku H guanidinium chloridem (cs) Native, denatured and reduced BSA enhancement of chronopotentiometric peak H by guanidinium chloride (en)
skos:prefLabel	Native, denatured and reduced BSA enhancement of chronopotentiometric peak H by guanidinium chloride Nativní, denaturovaný a redukovaný BSA. Zvýraznění chronopotenciometrického píku H guanidinium chloridem (cs) Native, denatured and reduced BSA enhancement of chronopotentiometric peak H by guanidinium chloride (en)
skos:notation	RIV/68081707:_____/08:00309822!RIV09-AV0-68081707
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/07/0490), P(GP202/07/P497), P(IAA500040513), P(LC06035), Z(AV0Z50040507), Z(AV0Z50040702)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Paleček, Emil Ostatná, Veronika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	381785
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/08:00309822
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	constant current chronopotentiometry; protein denaturation; guanidinium chloride (en)
http://linked.open.../riv/klicoveSlovo	protein denaturation constant current chronopotentiometry guanidinium chloride
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[DCB7679CEAA5]
http://linked.open...i/riv/nazevZdroje	Electrochimica acta
http://linked.open...in/vavai/riv/obor	AQ
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine. Protein and DNA-modified electrodes. New tools for biomedicine Interaction proteins with surfaces. New biophysical methods for analysis of tumor supressos protein p53 Wild type and mutant p53 tumor suppressor protein. Intermolecular interactions, changes in conformation and new micro-methods of their analysis
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Ostatná, Veronika Paleček, Emil
http://linked.open...ain/vavai/riv/wos	000254728900036
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Genome and epigenome: 1D and 3D Structure, Dynamics, Interactions with Proteins and Functions
issn	0013-4686
number of pages	8 (xsd:int)

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