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Description
| - Zkoumali jsme vliv inhibice endocytózy na lokalizaci proteinu Frizzled4 na hladinu proteinu Dishevelled a na signálování proteinu Wnt-3a. Inhibice endocytózy, ať už s využitím hyperosmolární sacharózy nebo deplece draslíkových iontů zvýšilo membránovou lokalizaci Frizzled4 a zároveň vyvolalo rychlou (1-2 h) a takřka kompletní (95%) degradaci endogenního Dishevelled, což v důsledku zabránilo přenosu signálu proteinu Wnt-3a k beta-cateninu. Tyto výsledky poskytují důkazy pro existenci molekulárního mechanismu, který může tvořit základ negativní zpětné vazby ve Wnt/Frizzled dráze, a který závisí na degradaci dishevelled. (cs)
- We investigated the effects of endocytosis inhibition on Frizzled4-green fluorescent protein (FZD4-GFP) localization, dishevelled levels and Wnt-3a signalling to beta-catenin. Hyperosmotic sucrose and K+ depletion increased the membrane localization of FZD4-GFP, and in parallel triggered fast (1-2 h) and almost complete (approx. 95%) degradation of endogenous dishevelled, which further prevented canonical signalling by Wnt-3a to beta-catenin. The data provide evidence for a molecular mechanism that could be a basis for a novel negative feedback loop within the Wnt/Frizzled pathway depending on dishevelled degradation.
- We investigated the effects of endocytosis inhibition on Frizzled4-green fluorescent protein (FZD4-GFP) localization, dishevelled levels and Wnt-3a signalling to beta-catenin. Hyperosmotic sucrose and K+ depletion increased the membrane localization of FZD4-GFP, and in parallel triggered fast (1-2 h) and almost complete (approx. 95%) degradation of endogenous dishevelled, which further prevented canonical signalling by Wnt-3a to beta-catenin. The data provide evidence for a molecular mechanism that could be a basis for a novel negative feedback loop within the Wnt/Frizzled pathway depending on dishevelled degradation. (en)
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Title
| - Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation
- Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation (en)
- Inhibice endocytózy blokuje přenos signálu Wnt na beta-katenin posílením degradace Dishevelled (cs)
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skos:prefLabel
| - Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation
- Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation (en)
- Inhibice endocytózy blokuje přenos signálu Wnt na beta-katenin posílením degradace Dishevelled (cs)
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skos:notation
| - RIV/68081707:_____/07:00082914!RIV07-AV0-68081707
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/07:00082914
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - beta-catenin; clathrin-mediated endocytosis; desensitization (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Acta physiologica Scandinavica
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Bryja, Vítězslav
- Schulte, G.
- Čajánek, L.
- Grahn, A.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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