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  • Provedli jsme více než 3500 PCR experimentů za různých podmínek s více než 400 fragmenty DNA o délce 4 - 150 nukleotidů. Zjistili jsme, že pro expanzi se zdá být nutné avšak ne dostačující opakování primární struktury. Dále jsme zjistili, že se (A+T) bohaté fragmenty prodlužují lépe než (G+C) bohaté fragmenty a že fragmenty bohaté na pyrimidinové báze se prodlužují lépe než fragmenty bohaté na purinové báze. Dalším důležitým faktorem pro expanzi je délka fragmentu a koncové nukleotidy. Jsou uváděny příklady, kdy relativně malá změna primární struktury způsobuje dramatické změny v expanzi. Tato práce má význam pro patologické prodlužování mikrosatelitů v lidském genomu stejně jako se týká genomové evoluce obecně. (cs)
  • We performed more than 3,500 PCRs under various conditions with more than 400 DNA fragments of 4-150 nucleotides in length. We found out that repetitiveness of the primary structure was mostly found to be necessary but not sufficient for the expansion. (A+T)-rich fragments expand better than (G+C)-rich ones and pyrimidine-rich fragments expand better than purine-rich fragments. Terminal nucleotides and the fragment length also are important for the expansion. examples are presented when relatively small alterations of the DNA primary structure caused a dramatic change in the expansion. The present work has implications for pathological expansions of microsatellites in the human genome as well as regarding the genome evolution in general.
  • We performed more than 3,500 PCRs under various conditions with more than 400 DNA fragments of 4-150 nucleotides in length. We found out that repetitiveness of the primary structure was mostly found to be necessary but not sufficient for the expansion. (A+T)-rich fragments expand better than (G+C)-rich ones and pyrimidine-rich fragments expand better than purine-rich fragments. Terminal nucleotides and the fragment length also are important for the expansion. examples are presented when relatively small alterations of the DNA primary structure caused a dramatic change in the expansion. The present work has implications for pathological expansions of microsatellites in the human genome as well as regarding the genome evolution in general. (en)
Title
  • Factors influencing DNA expansion in the course of polymerase chain reaction
  • Factors influencing DNA expansion in the course of polymerase chain reaction (en)
  • Faktory ovlivňující expanzi DNA během polymerázové řetězové reakce (cs)
skos:prefLabel
  • Factors influencing DNA expansion in the course of polymerase chain reaction
  • Factors influencing DNA expansion in the course of polymerase chain reaction (en)
  • Faktory ovlivňující expanzi DNA během polymerázové řetězové reakce (cs)
skos:notation
  • RIV/68081707:_____/07:00080841!RIV07-AV0-68081707
http://linked.open.../vavai/riv/strany
  • 65;82
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(NM7634), Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 421565
http://linked.open...ai/riv/idVysledku
  • RIV/68081707:_____/07:00080841
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • DNA expansion; PCR; microsatellites (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [F38D5AB01F7E]
http://linked.open...i/riv/nazevZdroje
  • Nucleosides Nucleotides & Nucleic Acids
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 26
http://linked.open...iv/tvurceVysledku
  • Kypr, Jaroslav
  • Vondrušková, Jitka
  • Pařízková, Naďa
http://linked.open...n/vavai/riv/zamer
issn
  • 1525-7770
number of pages
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