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Description
| - S využitím látky MK-886, inhibitoru proteinu aktivujícího 5-lipoxygenázu (5-LOX), byla studována role dráhy 5-LOX přeměny kyseliny arachidonové v diferenciaci indukované TNF-alpha u lidské buněčné leukemické linie HL-60. MK-886 posiloval zástavu buněčného cyklu a diferenciaci indukovanou TNF-alpha, ale tyto efekty jsou pravděpodobně nezávislé na inhibici 5-LOX, protože obecný inhibitor LOX, NDGA, neměl žádné účinky. Po kombinaci TNF-alpha a MK-886 byla významně potencována apoptóza, což částečně souviselo se změnami exprese proteinu Mc1-1. Signálování přes NF-ksíB nebo aktivace JNK nebyla MK-886 modulována. Tak, kromě apoptózy, MK-886 může zvyšovat i diferenciaci indukovanou TNF-alpha. (cs)
- We investigated the role of the 5-lipoxygenase (5-LOX) pathway of arachidonic acid metabolism in tumour necrosis factor-alpha (TNF-alpha)-induced differentiation of human leukemic HL-60 cells using MK-886, an inhibitor of 5-LOX activating protein. MK-886 augmented cell cycle arrest and differentiation induced by TNF-alpha; however, both effect were probably 5-LOX-independent, because a general LOX inhibitor, NDGA, had no effect. Apoptosis was significantly elevated after combined TNF-alpha and MK-886 treatment, which could be partially associated with changes of Mc1-1 protein expression. NF-ksíB signalling or activation of JNKs were not modulated by MK-886. Thus, in addition to apoptosis, MK-886 can enhance TNF-alpha-induced differentiation.
- We investigated the role of the 5-lipoxygenase (5-LOX) pathway of arachidonic acid metabolism in tumour necrosis factor-alpha (TNF-alpha)-induced differentiation of human leukemic HL-60 cells using MK-886, an inhibitor of 5-LOX activating protein. MK-886 augmented cell cycle arrest and differentiation induced by TNF-alpha; however, both effect were probably 5-LOX-independent, because a general LOX inhibitor, NDGA, had no effect. Apoptosis was significantly elevated after combined TNF-alpha and MK-886 treatment, which could be partially associated with changes of Mc1-1 protein expression. NF-ksíB signalling or activation of JNKs were not modulated by MK-886. Thus, in addition to apoptosis, MK-886 can enhance TNF-alpha-induced differentiation. (en)
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Title
| - MK-886 enhances tumour necrosis factor-alpha-induced differentiation and apoptosis
- MK-886 enhances tumour necrosis factor-alpha-induced differentiation and apoptosis (en)
- MK-886 zvyšuje diferenciaci a apoptózu indukovanou TNF-alpha (cs)
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skos:prefLabel
| - MK-886 enhances tumour necrosis factor-alpha-induced differentiation and apoptosis
- MK-886 enhances tumour necrosis factor-alpha-induced differentiation and apoptosis (en)
- MK-886 zvyšuje diferenciaci a apoptózu indukovanou TNF-alpha (cs)
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skos:notation
| - RIV/68081707:_____/06:00040473!RIV07-AV0-68081707
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA524/03/0766), Z(AV0Z50040507)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/06:00040473
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - cell differentiation; leukaemia; HL-60 cells (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Hofmanová, Jiřina
- Kozubík, Alois
- Vondráček, Jan
- Štika, Jiří
- Šimek, V.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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