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  • Přenos elektronů mezi kovovými elektrodami a ferro/ferricyanidem je plně potlačen při nízkých koncentracích iontů. Zde popisujeme nový jev vztahující se k této reakci: imobilizace thiolované jednořetězcové DNA na zlatých elektrodách uchovává tuto aktivitu na úrovni odpovídající vysokým iontovým silám. Naproti tomu hybridizace komplementární DNA s jednořetězcovou thiolovanou DNA potlačuje elektrokatalytický děj. Popsané děje mohou být využity k rozlišení jedno- a dvouřetězcové DNA a k semikvantitativnímu stanovování komplementární DNA ve vzorku. (cs)
  • Electron transfer between metal electrodes and ferro/ferricyanide is completely suppressed at low ionic concentration. We describe here a new phenomenon related to this reaction: an immobilisation of thiolated single-stranded DNA on gold electrodes retains this activity at low ionic strength up to the level corresponding to the high ionic strength. In contrast, a hybridisation of the complementary DNA with the thiolated single-stranded DNA followed by a binding onto the electrodes, attenuated the electrocatalytic effect. These effects can be used for discrimination between single-stranded DNA and double-stranded DNA and for semi-quantitative measurement of complementary DNA in a sample.
  • Electron transfer between metal electrodes and ferro/ferricyanide is completely suppressed at low ionic concentration. We describe here a new phenomenon related to this reaction: an immobilisation of thiolated single-stranded DNA on gold electrodes retains this activity at low ionic strength up to the level corresponding to the high ionic strength. In contrast, a hybridisation of the complementary DNA with the thiolated single-stranded DNA followed by a binding onto the electrodes, attenuated the electrocatalytic effect. These effects can be used for discrimination between single-stranded DNA and double-stranded DNA and for semi-quantitative measurement of complementary DNA in a sample. (en)
Title
  • Electrocatalytic activity of DNA on electrodes as an indication of hybridisation
  • Electrocatalytic activity of DNA on electrodes as an indication of hybridisation (en)
  • Elektrokatalytická aktivita DNA na elektrodách jako indikace hybridizace (cs)
skos:prefLabel
  • Electrocatalytic activity of DNA on electrodes as an indication of hybridisation
  • Electrocatalytic activity of DNA on electrodes as an indication of hybridisation (en)
  • Elektrokatalytická aktivita DNA na elektrodách jako indikace hybridizace (cs)
skos:notation
  • RIV/68081707:_____/06:00039040!RIV07-AV0-68081707
http://linked.open.../vavai/riv/strany
  • 1;6
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 473746
http://linked.open...ai/riv/idVysledku
  • RIV/68081707:_____/06:00039040
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • DNA; DNA-sensor; biosensor (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV
  • [63BA9836A86F]
http://linked.open...i/riv/nazevZdroje
  • Bioelectrochemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 68
http://linked.open...iv/tvurceVysledku
  • Dražan, Viktor
  • Mirsky, V. M.
  • Wolfbeis, O. S.
  • Zhi, Z.-L.
http://linked.open...n/vavai/riv/zamer
issn
  • 1567-5394
number of pages
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