About: Recognition of cisplatin-damaged DNA by p53 protein: Critical role of the p53 C-terminal domain

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About: Recognition of cisplatin-damaged DNA by p53 protein: Critical role of the p53 C-terminal domain Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Protein p53 can recognize DNA modified with cisplatin. We studied p53 binding to cisPt-DNA using p53 deletion mutants and via modulation of binding by changes of protein redox state. C-terminal domain (CTD) bound to the cisPt-DNA with higher affinity than to unmodified DNA. Constructs lacking C-terminal DNA binding site (CTDBS) exhibited small preference for cisPt-DNA. Oxidation of full length p53 did not affect its ability to recognize cisPt-DNA. Blocking of CTDBS by monoclonal antibody resulted in abolishement of the CTD binding to the cisPt-DNA. Our results demonstrate a crucial role of the basic region of the p53 CTD in the cisPt-DNA recognition. Protein p53 can recognize DNA modified with cisplatin. We studied p53 binding to cisPt-DNA using p53 deletion mutants and via modulation of binding by changes of protein redox state. C-terminal domain (CTD) bound to the cisPt-DNA with higher affinity than to unmodified DNA. Constructs lacking C-terminal DNA binding site (CTDBS) exhibited small preference for cisPt-DNA. Oxidation of full length p53 did not affect its ability to recognize cisPt-DNA. Blocking of CTDBS by monoclonal antibody resulted in abolishement of the CTD binding to the cisPt-DNA. Our results demonstrate a crucial role of the basic region of the p53 CTD in the cisPt-DNA recognition. (en) Protein p53 může rozpoznat DNA modifikovanou cisplatinou. Studovali jsme vazbu p53 na cisPt-DNA s využitím p53 delečních mutantů a modulací vazby změnou redox stavu proteinu. C-koncová doména (CTD) se vázala na cisPt-DNA s vyšší afinitou než k nemodifikované DNA. Konstrukty bez C-koncového DNA vazebného místa (CTDBS) vykazovaly malou preferenci k cisPt-DNA. Oxidace p53 o plné délce neovlivnila jeho schopnost rozpoznávat cisPt-DNA. Zablokování CTDBS monoklonální protilátkou vyústilo ve zrušení vazby CTD na cisPt-DNA. Naše výsledky demonstrují rozhodující úlohu bazické oblasti CTD proteinu p53 v rozpoznání cisPt-DNA. (cs)
Title	Recognition of cisplatin-damaged DNA by p53 protein: Critical role of the p53 C-terminal domain Recognition of cisplatin-damaged DNA by p53 protein: Critical role of the p53 C-terminal domain (en) Rozpoznání DNA poškozené cisplatinou proteinem p53: Rozhodující vliv C-koncové domény (cs)
skos:prefLabel	Recognition of cisplatin-damaged DNA by p53 protein: Critical role of the p53 C-terminal domain Recognition of cisplatin-damaged DNA by p53 protein: Critical role of the p53 C-terminal domain (en) Rozpoznání DNA poškozené cisplatinou proteinem p53: Rozhodující vliv C-koncové domény (cs)
skos:notation	RIV/68081707:_____/06:00039015!RIV07-AV0-68081707
http://linked.open.../vavai/riv/strany	477;484
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1QS500040581), P(GA204/02/0734), P(GA301/05/0416), P(NC7574), Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Brabec, Viktor Kašpárková, Jana Fojta, Miroslav Pivoňková, Hana Brázdová, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	496716
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/06:00039015
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cisplatin; anticancer drugs; tumor suppressor protein p53 (en)
http://linked.open.../riv/klicoveSlovo	cisplatin tumor suppressor protein p53 anticancer drugs
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1A1A5B918C26]
http://linked.open...i/riv/nazevZdroje	Biochemical and Biophysical Research Communications
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Metallodrugs, design and mechansim of action Development of novel therapeutic strategies through sensitising tumour cells to anti-cancer drugs by targeting p53-kinases and p53 homologues Interactions of tumor suppressor proteins with DNA. Roles of DNA conformation and protein modifications Recognizing DNA damage by Tumor Suppressor Proteins. Effects of Anti - Cancer Drugs.
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	339
http://linked.open...iv/tvurceVysledku	Brabec, Viktor Brázdová, Marie Fojta, Miroslav Kašpárková, Jana Pivoňková, Hana
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems
issn	0006-291X
number of pages	8 (xsd:int)

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