About: An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques

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About: An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The electroanalytical determination of avidin in solution, in a carbon paste, and in a transgenic maize extract was performed in acidic medium at a carbon paste electrode (CPE). The oxidative voltammetric signal resulting from the presence of tyrosine and tryptophan in avidin was observed using square-wave voltammetry. The process could be used to determine avidin concentrations up to 3 fM (100 amol in 3 ll drop) in solution, 700 fM (174 fmol in 250 ll solution) in an avidin-modified electrode, and 174 nM in a maize seed extract. The electroanalytical determination of avidin in solution, in a carbon paste, and in a transgenic maize extract was performed in acidic medium at a carbon paste electrode (CPE). The oxidative voltammetric signal resulting from the presence of tyrosine and tryptophan in avidin was observed using square-wave voltammetry. The process could be used to determine avidin concentrations up to 3 fM (100 amol in 3 ll drop) in solution, 700 fM (174 fmol in 250 ll solution) in an avidin-modified electrode, and 174 nM in a maize seed extract. (en) Elektroanalytické stanovení avidinu v roztoku, v uhlíkové pastě a v extraktu transgenních kukuřičných semen bylo provedeno v kyselém prostředí na uhlíkové pastové elektrodě. Oxidační voltametrický signál tyrosinu a tryptofanu v avidinu byl pozorován pomocí squar-wave voltametrie. Postup byl použit při stanovení avidinu až do koncentrace 3 fM (100 amol v 3 l kapce vzorku) v roztoku, 700 fM (174 fmol ve 250 ul vzorku) v elektrodě modifikované avidinem a v extraktu kukuřičných semen. (cs)
Title	An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques (en) Analýza avidinu, biotinu a jejich interakce na attomolární úrovni voltametrickými a chromatografickými technikami (cs)
skos:prefLabel	An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques (en) Analýza avidinu, biotinu a jejich interakce na attomolární úrovni voltametrickými a chromatografickými technikami (cs)
skos:notation	RIV/68081707:_____/05:00021190!RIV06-AV0-68081707
http://linked.open.../vavai/riv/strany	1167;1178
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/02/0422), P(GP525/04/P132), P(IAA1163201), P(LN00A081), Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Jelen, František Masařík, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	511883
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/05:00021190
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	avidin; biotin; avidin-biotin technology (en)
http://linked.open.../riv/klicoveSlovo	avidin biotin avidin-biotin technology
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[6DCA6B0BC30D]
http://linked.open...i/riv/nazevZdroje	Analytical and Bioanalytical Chemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Signaling pathways in plants New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Study of protection mechanisms for stress-induced by heavy metals Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	381
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Kizek, René Masařík, Michal Trnková, Libuše Jelen, František Klejdus, Bořivoj Mikelová, Radka Potěšil, David Bailey, Michele Howard, John A. Kramer, Karl J.
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems
issn	1618-2642
number of pages	12 (xsd:int)

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