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Description
| - Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1-393), and its C-terminal fragment, p53(320-393), with long (~3000 bp) dsDNA in linear and supercoiled forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA-protein filament, in which two DNA duplexes are linked (synapsed) via bound protein tetramers. The efficiency of the synapse, reflected in its length and the fraction of molecules exhibiting DNA-protein filaments, was significantly modulated by the molecular form of the protein and the topological state of the DNA. Possible implications for the sequestering of p53 in DNA-protein filaments are discussed.
- Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1-393), and its C-terminal fragment, p53(320-393), with long (~3000 bp) dsDNA in linear and supercoiled forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA-protein filament, in which two DNA duplexes are linked (synapsed) via bound protein tetramers. The efficiency of the synapse, reflected in its length and the fraction of molecules exhibiting DNA-protein filaments, was significantly modulated by the molecular form of the protein and the topological state of the DNA. Possible implications for the sequestering of p53 in DNA-protein filaments are discussed. (en)
- Pomocí elektronové mikroskopie byly analyzovány interakce proteinů p53 exprimovaných v bakteriích, celého proteinu p53, p53(1-393) a C-terminálního fragmentu p53(320-393), s dlouhou dsDNA (přibližně 3000 bp) v lineární a superhelikální formě obsahující či neobsahující proteinem p53 rozpoznávanou sekvenci (p53CON). Hlavním strukturním znakem vytvořených komplexů byl DNA -protein filament, ve kterém jsou duplexy DNA spojeny díky navázanému tetrametru proteinu. V práci byly diskutovány možné důsledky zachycení p53 prostřednictvím tvorby těchto DNA-proteinových filament. (cs)
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Title
| - Sequestering of p53 into DNA-protein filaments revealed by electron microscopy
- Zachycení p53 prostřednictvím filament DNA-protein zobrazené elektronovou (cs)
- Sequestering of p53 into DNA-protein filaments revealed by electron microscopy (en)
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skos:prefLabel
| - Sequestering of p53 into DNA-protein filaments revealed by electron microscopy
- Zachycení p53 prostřednictvím filament DNA-protein zobrazené elektronovou (cs)
- Sequestering of p53 into DNA-protein filaments revealed by electron microscopy (en)
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skos:notation
| - RIV/68081707:_____/05:00001223!RIV06-AV0-68081707
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA301/02/0831), P(GA301/99/D078), P(IAA4004110), P(IAA500040513), Z(AV0Z50040507)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/05:00001223
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - p53; electron microscopy; DNA-protein complex (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Brázdová, Marie
- Paleček, Emil
- Paleček, Jan
- Jovin, T. M.
- Cherny, Dmitry I.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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