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| Description
| - The level of differentiation could influence sensitivity of colonic epithelial cells to various stimuli. In our study, the effects of TNF-alpha, inhibitors of arachidonic acid (AA) metabolism (baicalein, BA; indomethacin, INDO; niflumic acid, NA; nordihydroguaiaretic acid, NDGA), and/or their combinations on undifferentiated or sodium butyrate (NaBt)-differentiated human colon adenocarcinoma HT-29 cells were compared. NaBt-treated cells became growth arrested (blocked in G(0)/G(1) phase of the cell cycle), and showed down-regulated Bcl-x(L) and up-regulated Bak proteins and increased expression of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). These cells were more perceptive to anti-proliferative and apoptotic effects of TNF-alpha. Both inhibitors of LOX (BA and NDGA) and COX (INDO and NA) in higher concentrations modulated cell cycle changes accompanying NaBt-induced differentiation and induced various level of cell death in undifferentiated and differentiated cells.
- The level of differentiation could influence sensitivity of colonic epithelial cells to various stimuli. In our study, the effects of TNF-alpha, inhibitors of arachidonic acid (AA) metabolism (baicalein, BA; indomethacin, INDO; niflumic acid, NA; nordihydroguaiaretic acid, NDGA), and/or their combinations on undifferentiated or sodium butyrate (NaBt)-differentiated human colon adenocarcinoma HT-29 cells were compared. NaBt-treated cells became growth arrested (blocked in G(0)/G(1) phase of the cell cycle), and showed down-regulated Bcl-x(L) and up-regulated Bak proteins and increased expression of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). These cells were more perceptive to anti-proliferative and apoptotic effects of TNF-alpha. Both inhibitors of LOX (BA and NDGA) and COX (INDO and NA) in higher concentrations modulated cell cycle changes accompanying NaBt-induced differentiation and induced various level of cell death in undifferentiated and differentiated cells. (en)
- Citlivost epiteliálních buněk kolonu k různým podnětům by mohla být ovlivňována stupněm diferenciace. V naší studii byly srovnávány účinky TNF-alfa, inhibitorů metabolismu kyseliny arachidonové (baicalein, BA; indomethacin, INDO; niflumová kyselina, NA; nordihydroguaiarová kyselina, NDGA) a jejich kombinací na nediferencované butyrátem sodným (NaBt) diferencované buňky lidského adenokarcinomu tlustého střeva HT-29. Buňky ovlivněné NaBt měly pozastavený růst (blok v G0/G1 fázi buněčného cyklu), vykazovaly snížení exprese Bcl-xL a zvýšení exprese Bak proteinů, rovněž zvýšenou expresi cyklooxygenázy-2 (COX-2) a 5-lipoxygenázy (5-LOX). Tyto buňky byly také vnímavější k antiproliferačním a apoptotickým účinkům TNF-alfa. Oba inhibitory LOX (BA a NDGA) a COX (INDO a NA) modulovaly ve vyšších koncentracích změny buněčného cyklu doprovázející diferenciaci navozenou NaBt a indukovaly různý stupeň buněčné smrti u nediferencovaných a diferencovaných buněk. (cs)
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| Title
| - The effects of TNF-alpha and inhibitors of arachidonic acid metabolism on human colon HT-29 cells depend on differentiation status
- Účinky TNF-alfa a inhibitorů metabolismu kyseliny arachidonové na lidské buňky tlustého střeva HT-29 závisí na stupni diferenciace (cs)
- The effects of TNF-alpha and inhibitors of arachidonic acid metabolism on human colon HT-29 cells depend on differentiation status (en)
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| skos:prefLabel
| - The effects of TNF-alpha and inhibitors of arachidonic acid metabolism on human colon HT-29 cells depend on differentiation status
- Účinky TNF-alfa a inhibitorů metabolismu kyseliny arachidonové na lidské buňky tlustého střeva HT-29 závisí na stupni diferenciace (cs)
- The effects of TNF-alpha and inhibitors of arachidonic acid metabolism on human colon HT-29 cells depend on differentiation status (en)
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| skos:notation
| - RIV/68081707:_____/04:00108496!RIV/2005/AV0/A17005/N
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA525/01/0419), P(GP524/02/P051), P(IBS5004009), Z(AV0Z5004920)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/04:00108496
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - colon cancer; cell differentiation; TNF-alpha (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Hofmanová, Jiřina
- Kozubík, Alois
- Souček, Karel
- Kovaříková, Martina
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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