About: Different nuclear localization of aberrant and normal chromosome 8 homologues in intact of NaBt differentiated HT-29 cells     Goto   Sponge   NotDistinct   Permalink

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Description
  • Lidská adenokarcinomová linie HT-29 je charakteristická početnými strukturálními aberacemi chromozómů. Buňky HT-29 mají za normálních podmínek nediferencovaný fenotyp, v přítomnosti různých induktorů diferenciace, např. butyrát sodný (NaBt), dochází k jejich přeměně na entorycyty, která je provázena řadou biochemických a morfologických změn. V těchto buňkách jsme prostřednictvím metody FISH v rámci homologického páru chromozomů 8 rozlišili aberantní a normální chromozóm. Analýzy jaderných parametrů ukázaly rozdíl v radiálním umístění mezi chromozómy. V porovnání s normálním chromozómem 8, byl aberantní chromozóm umístěn blíže k periferii jádra. Přítomnost induktoru NaBt neměla vliv na radiální distribuci normálního a aberantního chromozómu. (cs)
  • An accumulation of numerous chromosomal structural aberrations is a typical feature of the human colon adenocarcinoma cell line HT-29. In these cells the chromosome 8 territories were visualized using fluorescence in situ hybridisation (FISH), which enabled to distinguish the aberrant chromosome 8 homologue from the normal one. Analyses of nuclear parameters revealed differences in radial positioning between chromosome homologues analysed. In comparison with an normal chromosome 8, the aberrant chromosome 8 territory was positioned closer to the nuclear periphery. Under standard conditions HT-29 cells displayed relatively undifferentiated phenotype but incubation of these cells in the presence of sodium butyrate (5mM) induced biochemical and morphological changes that are typical of well-differentiated phenotype of enterocytes.
  • An accumulation of numerous chromosomal structural aberrations is a typical feature of the human colon adenocarcinoma cell line HT-29. In these cells the chromosome 8 territories were visualized using fluorescence in situ hybridisation (FISH), which enabled to distinguish the aberrant chromosome 8 homologue from the normal one. Analyses of nuclear parameters revealed differences in radial positioning between chromosome homologues analysed. In comparison with an normal chromosome 8, the aberrant chromosome 8 territory was positioned closer to the nuclear periphery. Under standard conditions HT-29 cells displayed relatively undifferentiated phenotype but incubation of these cells in the presence of sodium butyrate (5mM) induced biochemical and morphological changes that are typical of well-differentiated phenotype of enterocytes. (en)
Title
  • Different nuclear localization of aberrant and normal chromosome 8 homologues in intact of NaBt differentiated HT-29 cells
  • Rozdílná jaderná lokalizace aberantních a normálních homologů chromosomu 8 v intaktních nebo NaBt diferencovaných buňkách HT-29 (cs)
  • Different nuclear localization of aberrant and normal chromosome 8 homologues in intact of NaBt differentiated HT-29 cells (en)
skos:prefLabel
  • Different nuclear localization of aberrant and normal chromosome 8 homologues in intact of NaBt differentiated HT-29 cells
  • Rozdílná jaderná lokalizace aberantních a normálních homologů chromosomu 8 v intaktních nebo NaBt diferencovaných buňkách HT-29 (cs)
  • Different nuclear localization of aberrant and normal chromosome 8 homologues in intact of NaBt differentiated HT-29 cells (en)
skos:notation
  • RIV/68081707:_____/04:00108492!RIV/2005/AV0/A17005/N
http://linked.open.../vavai/riv/strany
  • 24;27
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA202/04/0907), P(IAA5004306), P(IBS5004010), P(KSK5052113), P(ME 565), P(NC6987), Z(AV0Z5004920)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 560560
http://linked.open...ai/riv/idVysledku
  • RIV/68081707:_____/04:00108492
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • chromosome 8 homologues; NaBt differentiated HT-29 cells (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [1B414DEA1F75]
http://linked.open...v/mistoKonaniAkce
  • Brno
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • Biophysics of the Genome: Proceedings of the Workshop Held at Brno
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Bártová, Eva
  • Kozubek, Stanislav
  • Harničarová, Andrea
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Masarykova univerzita v Brně
https://schema.org/isbn
  • 80-210-3560-9
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