About: Cyclic voltammetric study of the redox system of glutathione using the disulfide bond reductant tris(2-carboxyethyl)phosphine

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Glutathione in the reduced (GSH) and oxidized (GSSG) forms was studied by cyclic voltammetry. Tris(2-carboxyethyl)phosphine (TCEP) as the disulfide bond reductant and/or hydrogen peroxide as the sulfhydryl group oxidant were used. Cyclic voltammetry measurements, following the redox state of glutathione, were performed on a hanging mercury drop electrode (HMDE) in borate buffer (pH 9.2). It was shown that in aqueous solutions TCEP was able to reduce disulfide groups smoothly and quantitatively. The TCEP response at -0.25 V vs. Ag/AgCl/3 M KCl did not disturb the signals of the thiol/disulfide redox couple. Glutathione in the reduced (GSH) and oxidized (GSSG) forms was studied by cyclic voltammetry. Tris(2-carboxyethyl)phosphine (TCEP) as the disulfide bond reductant and/or hydrogen peroxide as the sulfhydryl group oxidant were used. Cyclic voltammetry measurements, following the redox state of glutathione, were performed on a hanging mercury drop electrode (HMDE) in borate buffer (pH 9.2). It was shown that in aqueous solutions TCEP was able to reduce disulfide groups smoothly and quantitatively. The TCEP response at -0.25 V vs. Ag/AgCl/3 M KCl did not disturb the signals of the thiol/disulfide redox couple. (en) Glutathion v jeho redukované (GSH) a oxidované formě (GSSG) byl studován pomocí cyklické voltametrie. V experimentech byl využit TCPE pro redukci disulfidické vazby a peroxid vodíku pro oxidaci sulfhydrylové skupiny. CV měření byla provedena na HMDE v borátovém pufru, pH 9,2. Zjistili jsme, že ve vodném prostředí TCEP redukuje snadno a kvantitativně disulfidickou vazbu. Signál TCEP byl pozorován při potenciálu -0,25 V (proti Ag/AgCl/KCl elektrodě) a neovlivňoval signál redox páru thiol/disulfid. V práci je diskutován vznik signálů glutathionu GSH (při -0,44 a -0,37V) a GSSG (při -0,69 a -0,40 V). Stabilizace redukovaného stavu bílkovin a peptidů je velmi důležitá pro monitorování interakcí bílkovina-bílkovina, bílkovina-DNA a bílkovina-xenobiotika. (cs)
Title	Cyclic voltammetric study of the redox system of glutathione using the disulfide bond reductant tris(2-carboxyethyl)phosphine Cyclic voltammetric study of the redox system of glutathione using the disulfide bond reductant tris(2-carboxyethyl)phosphine (en) Využití cyklické voltametrie pro studium redoxního systému glutathionu a disulfidické vazby redukčního činidla tris(2-karboxyetyl) fosfínu (TCPE) (cs)
skos:prefLabel	Cyclic voltammetric study of the redox system of glutathione using the disulfide bond reductant tris(2-carboxyethyl)phosphine Cyclic voltammetric study of the redox system of glutathione using the disulfide bond reductant tris(2-carboxyethyl)phosphine (en) Využití cyklické voltametrie pro studium redoxního systému glutathionu a disulfidické vazby redukčního činidla tris(2-karboxyetyl) fosfínu (TCPE) (cs)
skos:notation	RIV/68081707:_____/04:00104657!RIV/2005/AV0/A17005/N
http://linked.open.../vavai/riv/strany	19;24
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/02/0422), P(IAA1163201), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Vacek, Jan Jelen, František Kizek, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	559163
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/04:00104657
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	voltammetry; hanging mercury drop electrode (HMDE); glutathione (GSH, GSSG) (en)
http://linked.open.../riv/klicoveSlovo	GSSG) glutathione (GSH hanging mercury drop electrode (HMDE) voltammetry
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[D526AFA9232C]
http://linked.open...i/riv/nazevZdroje	Bioelectrochemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	63
http://linked.open...iv/tvurceVysledku	Kizek, René Vacek, Jan Jelen, František Trnková, L.
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	1567-5394
number of pages	6 (xsd:int)

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