About: Electrochemical detection of specific DNA sequences from PCR amplicons on carbon and mercury electrodes using Meldola's Blue as an intercalator

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About: Electrochemical detection of specific DNA sequences from PCR amplicons on carbon and mercury electrodes using Meldola's Blue as an intercalator Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Byly popsány elektrochemické parametry vazby DNA-Meldola blue (MDB) (7-dimethyl-amino-1,2-benzophenoxazinium) jak na visící rtuťové elektrodě (HMDE), tak na uhlíkové pastové elektrodě (CPE). S využitím přenosové voltametrie a sledováním poklesu signálu MDB byly pozorovány interakce MDB jak s dvouřetězcovou, tak jednořetězcovou DNA imobilizovanou na HMDE nebo CPE. Rozdíly ve výšce voltametrického signálu MDB byly rovněž využity ke sledování hybridizace u syntetických oligonukleotidů a rozdílů mezi ss DNA a ds DNA při interakci s MDB. (cs) The electrochemical parameters for 7--dimethyl--amino--1,2-- benzophenoxazinium Meldola's Blue, (MDB) on binding to DNA at both a hanging mercury drop electrode (HMDE) and carbon paste electrode (CPE) are described. MDB, which interacts with immobilized calf thymus DNA, was detected using double stranded DNA modified HMDE or CPE (dsDNA modified HMDE or CPE), bare HMDE or CPE and single stranded DNA modified HMDE or CPE (ssDNA modified HMDE or CPE) in combination with adsorptive transfer stripping voltammetry (AdTSV) techniques and decreased peak currents were observed. The electrochemical parameters for 7--dimethyl--amino--1,2-- benzophenoxazinium Meldola's Blue, (MDB) on binding to DNA at both a hanging mercury drop electrode (HMDE) and carbon paste electrode (CPE) are described. MDB, which interacts with immobilized calf thymus DNA, was detected using double stranded DNA modified HMDE or CPE (dsDNA modified HMDE or CPE), bare HMDE or CPE and single stranded DNA modified HMDE or CPE (ssDNA modified HMDE or CPE) in combination with adsorptive transfer stripping voltammetry (AdTSV) techniques and decreased peak currents were observed. (en)
Title	Electrochemical detection of specific DNA sequences from PCR amplicons on carbon and mercury electrodes using Meldola's Blue as an intercalator Elektrochemická detekce specifických sekvencí DNA s využitím indikátoru Meldola blue (cs) Electrochemical detection of specific DNA sequences from PCR amplicons on carbon and mercury electrodes using Meldola's Blue as an intercalator (en)
skos:prefLabel	Electrochemical detection of specific DNA sequences from PCR amplicons on carbon and mercury electrodes using Meldola's Blue as an intercalator Elektrochemická detekce specifických sekvencí DNA s využitím indikátoru Meldola blue (cs) Electrochemical detection of specific DNA sequences from PCR amplicons on carbon and mercury electrodes using Meldola's Blue as an intercalator (en)
skos:notation	RIV/68081707:_____/04:00104656!RIV/2005/AV0/A17005/N
http://linked.open.../vavai/riv/strany	523;533
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Jelen, František
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	562505
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/04:00104656
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA; biosensor; Meldola's Blue (en)
http://linked.open.../riv/klicoveSlovo	DNA Meldola's Blue biosensor
http://linked.open...odStatuVydavatele	TR - Turecká republika
http://linked.open...ontrolniKodProRIV	[2EB28A43C064]
http://linked.open...i/riv/nazevZdroje	Turkish Journal of Chemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Jelen, František Erdem, A. Kara, P. Kerman, K. Karadeniz, H. Özkan, D. Özkan, Z. Özsöz, M.
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	1010-7614
number of pages	11 (xsd:int)

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