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| rdf:type
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| Description
| - DNA molecules of pUC19, pBR322 and PhiX174 were irradiated by various doses of UV light and the irradiated molecules were cleaved by about two dozen type II restrictases. The irradiation generally blocked the cleavage in a dose-dependent way. In accordance with previous studies, the (A + T)-richness and the (PyPy) dimer content of the restriction site belongs among the factors that on average, cause an increase in the resistance of UV damaged DNA to the restrictase cleavage. However, we observed strong effects of UV irradiation even with (G + C)-rich and (PyPy)-poor sites. In addition, sequences flanking the restriction site influenced the protection in some cases (e.g. HindIII), but not in others (e.g. SalI), whereas neoschizomer couples SmaI and AvaI, or SacI and Ecl136II, cleaved the UV-irradiated DNA similarly. Hence the intrastrand thymine dimers located in the recognition site are not the only photoproduct blocking the restrictases.
- DNA molecules of pUC19, pBR322 and PhiX174 were irradiated by various doses of UV light and the irradiated molecules were cleaved by about two dozen type II restrictases. The irradiation generally blocked the cleavage in a dose-dependent way. In accordance with previous studies, the (A + T)-richness and the (PyPy) dimer content of the restriction site belongs among the factors that on average, cause an increase in the resistance of UV damaged DNA to the restrictase cleavage. However, we observed strong effects of UV irradiation even with (G + C)-rich and (PyPy)-poor sites. In addition, sequences flanking the restriction site influenced the protection in some cases (e.g. HindIII), but not in others (e.g. SalI), whereas neoschizomer couples SmaI and AvaI, or SacI and Ecl136II, cleaved the UV-irradiated DNA similarly. Hence the intrastrand thymine dimers located in the recognition site are not the only photoproduct blocking the restrictases. (en)
- DNA molekul pUC19, pBR322 a PhiX174 byla ozářena různými dávkami ultrafialového (UV) světla a následně byla štěpena více než dvaceti restriktasami typu II. Štěpení bylo obecně inhibováno ozářením, v závislosti na velikosti dávky. V souladu s dřívějšími studiemi, mezi faktory, které jako celek způsobují zvýšení odolnosti DNA (poškozené UV světlem) vůči restriktasovému štěpení, patří bohaté zastoupení (A+T) párů a obsah dimérů (PyPy). Nicméně jsme pozorovali silný vliv ozáření UV světlem i na úseky bohaté (G+C) páry a chudé na diméry (PyPy). Navíc, sekvence obklopující restrikční místo v některých případech ochranu před štěpením ovlivňovaly (např. HindIII), zatímco v jiných nikoliv (např. SalI). Přitom neoschizomerové páry SmaI a AvaI, nebo SacI a Ecl136II štěpily DNA ozářenou UV světlem podobně. Tudíž vnitrořetězcové thyminové diméry uvnitř rekogničního místa nejsou jediným fotoproduktem, blokujícím restriktasové štěpení. (cs)
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| Title
| - Factors influencing resistance of UV-irradiated DNA to the restriction endonuclease cleavage
- Factors influencing resistance of UV-irradiated DNA to the restriction endonuclease cleavage (en)
- Faktory ovlivňující odolnost DNA ozářené ultrafialovým světlem vůči štěpení restrikčními endonukleasami (cs)
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| skos:prefLabel
| - Factors influencing resistance of UV-irradiated DNA to the restriction endonuclease cleavage
- Factors influencing resistance of UV-irradiated DNA to the restriction endonuclease cleavage (en)
- Faktory ovlivňující odolnost DNA ozářené ultrafialovým světlem vůči štěpení restrikčními endonukleasami (cs)
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| skos:notation
| - RIV/68081707:_____/04:00104655!RIV/2005/AV0/A17005/N
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(IAA1004201), P(IAA1004301), P(IAA5004802), Z(AV0Z5004920)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/04:00104655
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - DNA; structure; damage (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - International Journal of Biological Macromolecules
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Kejnovský, Eduard
- Kypr, Jaroslav
- Nejedlý, Karel
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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