Attributes | Values |
---|
rdf:type
| |
Description
| - Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants.
- Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants. (en)
|
Title
| - Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation.
- Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. (en)
|
skos:prefLabel
| - Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation.
- Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. (en)
|
skos:notation
| - RIV/68081707:_____/02:17023186!RIV/2003/GA0/A17003/N
|
http://linked.open.../vavai/riv/strany
| |
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(GA524/99/0694), P(IBS5004009), P(KSK5011112), Z(AV0Z5004920)
|
http://linked.open...iv/cisloPeriodika
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/02:17023186
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - leukaemia cell line HL-60; electroporation; gene transfection (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...odStatuVydavatele
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/nazevZdroje
| |
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...ocetUcastnikuAkce
| |
http://linked.open...nichUcastnikuAkce
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...v/svazekPeriodika
| |
http://linked.open...iv/tvurceVysledku
| - Bryja, Vítězslav
- Hofmanová, Jiřina
- Kozubík, Alois
- Pacherník, Jiří
- Janík, Robert
|
http://linked.open...n/vavai/riv/zamer
| |
issn
| |
number of pages
| |