About: Determination of nanogram quantities of osmium-labeled single stranded DNA by differential pulse stripping voltammetry.

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About: Determination of nanogram quantities of osmium-labeled single stranded DNA by differential pulse stripping voltammetry. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Earlier, we showed that using differential pulse cathodic stripping voltammetry with hanging mercury drop electrode (HMDE), single-stranded (ss) DNA modified with osmium tetroxide, pyridine reagent (Os, py) can be determined at concentrations down to about 10-5 ng/ml. Here, we show that by exchanging Os, py for osmium tetroxide, 2,2 -bipyridine (Os, bipy) and decreasing the pH of the background electrolyte from neutrality to about pH 4, ssDNA can be determined at concentrations lower by one order of magnitude. Determination of DNA at such low concentrations may find use in various areas of molecular biology and in biotechnologies, including the development of DNA sensors. Earlier, we showed that using differential pulse cathodic stripping voltammetry with hanging mercury drop electrode (HMDE), single-stranded (ss) DNA modified with osmium tetroxide, pyridine reagent (Os, py) can be determined at concentrations down to about 10-5 ng/ml. Here, we show that by exchanging Os, py for osmium tetroxide, 2,2 -bipyridine (Os, bipy) and decreasing the pH of the background electrolyte from neutrality to about pH 4, ssDNA can be determined at concentrations lower by one order of magnitude. Determination of DNA at such low concentrations may find use in various areas of molecular biology and in biotechnologies, including the development of DNA sensors. (en)
Title	Determination of nanogram quantities of osmium-labeled single stranded DNA by differential pulse stripping voltammetry. Determination of nanogram quantities of osmium-labeled single stranded DNA by differential pulse stripping voltammetry. (en)
skos:prefLabel	Determination of nanogram quantities of osmium-labeled single stranded DNA by differential pulse stripping voltammetry. Determination of nanogram quantities of osmium-labeled single stranded DNA by differential pulse stripping voltammetry. (en)
skos:notation	RIV/68081707:_____/02:17023180!RIV/2003/GA0/A17003/N
http://linked.open.../vavai/riv/strany	199;121
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/00/D049), P(GV204/97/K084), P(IAA4004108), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	1/2
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Paleček, Emil Havran, Luděk Fojta, Miroslav Kizek, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	642741
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/02:17023180
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	differential pulse stripping voltammetry; microdetermination of DNA; chemical modification of DNA (en)
http://linked.open.../riv/klicoveSlovo	chemical modification of DNA differential pulse stripping voltammetry microdetermination of DNA
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[A22BB9D4500E]
http://linked.open...i/riv/nazevZdroje	Bioelectrochemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Influence of chemical modification of DNA and synthetic oligonucleotides on their electrochemical behaviour Electrodes modified with nucleic acids and proteins.New tools in biochemical and biomedical research. Development of electrochemical biodetectors for DNA damage
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Havran, Luděk Kizek, René Paleček, Emil Fojta, Miloslav
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	1567-5394
number of pages	3 (xsd:int)

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