About: Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper.

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About: Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Electrochemical study is dealing with highly sensitive label-free techniques of DNA determination. DNA can be determined using copper solid amalgam electrodes or hanging mercury drop electrodes in the presence of copper. Small volumes of 5 - 3 muL of acid-treated DNA can easily be analyzed, thus making possible the determination of picogram and subpicogram amounts of DNA corresponding to attomole and subattomole quantities of 1000-base pair DNA. Application of this determination in DNA hybridization detection is demonstrated using surface H for the hybridization (superparamagnetic beads with covalently attached DNA probe) and the mercury electrodes only for the determination of DNA selectively captured at surface H. Electrochemical study is dealing with highly sensitive label-free techniques of DNA determination. DNA can be determined using copper solid amalgam electrodes or hanging mercury drop electrodes in the presence of copper. Small volumes of 5 - 3 muL of acid-treated DNA can easily be analyzed, thus making possible the determination of picogram and subpicogram amounts of DNA corresponding to attomole and subattomole quantities of 1000-base pair DNA. Application of this determination in DNA hybridization detection is demonstrated using surface H for the hybridization (superparamagnetic beads with covalently attached DNA probe) and the mercury electrodes only for the determination of DNA selectively captured at surface H. (en)
Title	Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper. Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper. (en)
skos:prefLabel	Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper. Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper. (en)
skos:notation	RIV/68081707:_____/02:17023010!RIV/2003/GA0/A17003/N
http://linked.open.../vavai/riv/strany	4788;4793
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/02/0422), P(IAA1163201), P(IBS5004107), Z(AV0Z4040901), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	18
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Paleček, Emil Jelen, František Kouřilová, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	651439
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/02:17023010
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	hybridization; adenine; accumulation (en)
http://linked.open.../riv/klicoveSlovo	accumulation hybridization adenine
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F50FDBC7730F]
http://linked.open...i/riv/nazevZdroje	Analytical Chemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Application of biophysical methods in biotechnological and clinical praxis Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	74
http://linked.open...iv/tvurceVysledku	Novotný, Ladislav Yosypchuk, Bogdan Jelen, František Paleček, Emil Kouřilová, Alena
http://linked.open...n/vavai/riv/zamer	Objasňování zákonitostí vztahů struktury a vlastností - zejména dynamiky a chemické reaktivity - látek a molekulárních systémů Biophysical properties of living systems and their changes under the influence of environmental factors
issn	0003-2700
number of pages	6 (xsd:int)

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