About: Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta)     Goto   Sponge   NotDistinct   Permalink

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  • Three methods of algal assays with green alga Chlorella kessleri - the standard assay, microassay, and the proposed fluorescence assay - were compared from the point of view of reliability of EC50 detection, the minimum required time for the detection, sensitivity of individual measurement, i.e. at which cell density the particular assay can be used for EC50 estimation, and the time stability of the EC50 values. No significant differences in EC50 between the assay type and the inoculation methods were observed with the exception of the significant difference between ECc50-72 in the standard assay and ECr50 in the microassay in the standard inoculation. The ECf50 derived from variable fluorescence measurement values correspond to EC50 values derived from the growth rates. Fluorescence measurement revealed the toxic effect after 24 h of exposure (24 h before the other bioassays) at lower cell density (50%).
  • Three methods of algal assays with green alga Chlorella kessleri - the standard assay, microassay, and the proposed fluorescence assay - were compared from the point of view of reliability of EC50 detection, the minimum required time for the detection, sensitivity of individual measurement, i.e. at which cell density the particular assay can be used for EC50 estimation, and the time stability of the EC50 values. No significant differences in EC50 between the assay type and the inoculation methods were observed with the exception of the significant difference between ECc50-72 in the standard assay and ECr50 in the microassay in the standard inoculation. The ECf50 derived from variable fluorescence measurement values correspond to EC50 values derived from the growth rates. Fluorescence measurement revealed the toxic effect after 24 h of exposure (24 h before the other bioassays) at lower cell density (50%). (en)
Title
  • Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta)
  • Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta) (en)
skos:prefLabel
  • Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta)
  • Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta) (en)
skos:notation
  • RIV/67985939:_____/10:00357655!RIV11-MSM-67985939
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M0571), Z(AV0Z60050516)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 283906
http://linked.open...ai/riv/idVysledku
  • RIV/67985939:_____/10:00357655
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • bioassay; variable chlorophyll fluorescence; Chlorella kessleri (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [15ECC33DAB99]
http://linked.open...i/riv/nazevZdroje
  • Environmental Toxicology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 25
http://linked.open...iv/tvurceVysledku
  • Kvíderová, Jana
http://linked.open...ain/vavai/riv/wos
  • 000284683800003
http://linked.open...n/vavai/riv/zamer
issn
  • 1520-4081
number of pages
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