About: Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We applied CLSM and Two-Photon Excitation (TPE) microscopy techniques to visualize testate amoebae (TA), their test, morphology and physiology. TA are usually examined using SEM and E-SEM. It is not possible to acquire images of living TA and physiology of the amoeba inside the test by SEM or E-SEM. The goal of the study was to examine the potential of CLSM and TPE for imaging of TA. We tried 17 fluorescent dyes to label different structures of TA. CLSM enabled us to acquire images of TA from depths up to 40 μm, whereas TPE was able to penetrate to 60 μm. Stereological methods were employed to estimate the volume of the biomass of TA visualized by CLSM We applied CLSM and Two-Photon Excitation (TPE) microscopy techniques to visualize testate amoebae (TA), their test, morphology and physiology. TA are usually examined using SEM and E-SEM. It is not possible to acquire images of living TA and physiology of the amoeba inside the test by SEM or E-SEM. The goal of the study was to examine the potential of CLSM and TPE for imaging of TA. We tried 17 fluorescent dyes to label different structures of TA. CLSM enabled us to acquire images of TA from depths up to 40 μm, whereas TPE was able to penetrate to 60 μm. Stereological methods were employed to estimate the volume of the biomass of TA visualized by CLSM (en)
Title	Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae (en)
skos:prefLabel	Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae (en)
skos:notation	RIV/67985939:_____/10:00354019!RIV11-MSM-67985939
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA102/08/0691), P(GA304/09/0733), P(LC06063), Z(AV0Z50110509), Z(AV0Z60050516)
http://linked.open...iv/cisloPeriodika	Suppl.2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Machač, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Botanický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	251841
http://linked.open...ai/riv/idVysledku	RIV/67985939:_____/10:00354019
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	testate amoebae; confocal microscopy; two-photon microscopy (en)
http://linked.open.../riv/klicoveSlovo	testate amoebae confocal microscopy two-photon microscopy
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[FAA67B5856FD]
http://linked.open...i/riv/nazevZdroje	Microscopy and Microanalysis
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Visualization and processing of 3D images captured by a confocal microscope using graphics processor unit programming and virtual reality Three-dimensional arrangement and remodelation of the capillary bed of placentas from pregnancies complicated by type I diabetes mellitus Flurescence microscopy in biological and medical research
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	16
http://linked.open...iv/tvurceVysledku	Burdíková, Zuzana Kubínová, Lucie Čapek, Martin Ostašov, Pavel Machač, Jiří Mitchell, E.A.D.
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka Struktura, funkce a evoluce biodiversity fotoautotrofních organismů a hub: původ a příčiny jejich proměnlivosti, dynamika populací, společenstev a ekosystémů; využití vybraných výsledků k rozvoji Průhonického parku
issn	1431-9276
number of pages	2 (xsd:int)

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