About: Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to their multilineage differentiation capacity NCCs offer a cell source for regenerative medicine. Both these aspects make NCC biology an important issue to study, which can currently be addressed using methodologies based on pluripotent stem cells. Here we contributed to understanding the biology of human NCCs by refining the protocol for differentiation/propagation of NCC-like cells from human embryonic stem cells and by characterizing the molecular and functional phenotype of such cells. Most importantly, we improved formulation of media for NCC culture, we found that poly-L-ornithine combined with fibronectin provide good support for NCC growth, we unravelled the tendency of cultured NCCs to maintain heterogeneity of CD271 expression, and we showed that NCCs derived here possess the capacity to react to BMP4 signals by dramatically up-regulating MSX1, which is linked to odontogenesis. Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to their multilineage differentiation capacity NCCs offer a cell source for regenerative medicine. Both these aspects make NCC biology an important issue to study, which can currently be addressed using methodologies based on pluripotent stem cells. Here we contributed to understanding the biology of human NCCs by refining the protocol for differentiation/propagation of NCC-like cells from human embryonic stem cells and by characterizing the molecular and functional phenotype of such cells. Most importantly, we improved formulation of media for NCC culture, we found that poly-L-ornithine combined with fibronectin provide good support for NCC growth, we unravelled the tendency of cultured NCCs to maintain heterogeneity of CD271 expression, and we showed that NCCs derived here possess the capacity to react to BMP4 signals by dramatically up-regulating MSX1, which is linked to odontogenesis. (en)
Title	Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells (en)
skos:prefLabel	Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells (en)
skos:notation	RIV/67985904:_____/14:00436649!RIV15-GA0-67985904
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.100/02/0123), P(GAP304/11/1418)
http://linked.open...iv/cisloPeriodika	Suppl 1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hampl, Aleš Buchtová, Marcela Matalová, Eva Švandová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	40329
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/14:00436649
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	stem cell differentiation; neural crest; odontogenesis; cell therapies (en)
http://linked.open.../riv/klicoveSlovo	odontogenesis neural crest cell therapies stem cell differentiation
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[FDAB81831AC1]
http://linked.open...i/riv/nazevZdroje	Folia Biologica
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Differentiation of human embryonic stem cells into odontogenic lineages St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Buchtová, Marcela Hampl, Aleš Souček, Karel Matalová, Eva Jaroš, J. Švandová, Eva Fedr, Radek Vinarsky, V. Křivánek, J. Hajda, S. Králik, J.
http://linked.open...ain/vavai/riv/wos	000343275800005
issn	0015-5500
number of pages	9 (xsd:int)

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