About: Identification of Bifidobacterium strains from faeces of lambs

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Bifidobacteria were enumerated and isolated from the faeces of lambs aged between 25 and 40 days using modified trypticase-phytone-yeast extract (TPY) agar supplemented with mupirocin and acetic acid. Nineteen strains were characterised and divided into 6 groups according their fermentation characteristics obtained by API 50 CHL and RAPID ID 32 tests and by randomly amplified polymorphic DNA (RAPD) profiles. One representative strain of each group was identified by sequencing by 16S rRNA and hsp60 genes sequencing as B. animalis ssp. animalis, B. choerinum, B. pseudolongum ssp. globosum, B. pseudocatenulatum. Examination of the DNA fingerprinting showed a genetic diversity between strains of B. animalis ssp. animalis and B. choerinum. Our result shows that some bifidobacterial species should not be host-specific or have a broad host spectrum. As bifidobacteria were determined as numerous bacterial group in the intestine of lambs on a milk diet (8.32 +/- 0.71 log CFU/g, mean count +/- S.D., n = 30), there is a reasonable presumption that bifidobacteria could be an effective probiotics for lambs. Identification techniques used in this study were found to be suitable for the classification of lamb origin bifidobacteria. Bifidobacteria were enumerated and isolated from the faeces of lambs aged between 25 and 40 days using modified trypticase-phytone-yeast extract (TPY) agar supplemented with mupirocin and acetic acid. Nineteen strains were characterised and divided into 6 groups according their fermentation characteristics obtained by API 50 CHL and RAPID ID 32 tests and by randomly amplified polymorphic DNA (RAPD) profiles. One representative strain of each group was identified by sequencing by 16S rRNA and hsp60 genes sequencing as B. animalis ssp. animalis, B. choerinum, B. pseudolongum ssp. globosum, B. pseudocatenulatum. Examination of the DNA fingerprinting showed a genetic diversity between strains of B. animalis ssp. animalis and B. choerinum. Our result shows that some bifidobacterial species should not be host-specific or have a broad host spectrum. As bifidobacteria were determined as numerous bacterial group in the intestine of lambs on a milk diet (8.32 +/- 0.71 log CFU/g, mean count +/- S.D., n = 30), there is a reasonable presumption that bifidobacteria could be an effective probiotics for lambs. Identification techniques used in this study were found to be suitable for the classification of lamb origin bifidobacteria. (en)
Title	Identification of Bifidobacterium strains from faeces of lambs Identification of Bifidobacterium strains from faeces of lambs (en)
skos:prefLabel	Identification of Bifidobacterium strains from faeces of lambs Identification of Bifidobacterium strains from faeces of lambs (en)
skos:notation	RIV/67985904:_____/12:00387185!RIV13-GA0-67985904
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA523/08/1091), P(GD525/08/H060)
http://linked.open...iv/cisloPeriodika	1-3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Killer, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	140280
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/12:00387185
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bifidobacterium; identification; lambs (en)
http://linked.open.../riv/klicoveSlovo	identification lambs bifidobacterium
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[8AD3870CF717]
http://linked.open...i/riv/nazevZdroje	Small Ruminant Research
http://linked.open...in/vavai/riv/obor	GH
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Synbiotics and plant extracts in human and animal nutrition Administration of bifidobacteria to young ruminants
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	105
http://linked.open...iv/tvurceVysledku	Killer, Jiří Rada, V. Ročková, Š. Vlková, E. Bunešová, V.
http://linked.open...ain/vavai/riv/wos	000305100900058
issn	0921-4488
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.smallrumres.2011.12.004

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