About: PCR detection of uncultured rumen bacteria

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	16S rRNA sequences of ruminal uncultured bacterial clones from public databases were phylogenetically examined. The sequences were found to form two unique clusters not affiliated with any known bacterial species: cluster of unidentified sequences of free floating rumen fluid uncultured bacteria (FUB) and cluster of unidentified sequences of bacteria associated with rumen epithelium (AUB). A set of PCR primers targeting 16S rRNA of ruminal free uncultured bacteria and rumen epithelium adhering uncultured bacteria was designed based on these sequences. FUB primers were used for relative quantification of uncultured bacteria in ovine rumen samples. The effort to increase the population size of FUB group has been successful in sulfate reducing broth and culture media supplied with cellulose. 16S rRNA sequences of ruminal uncultured bacterial clones from public databases were phylogenetically examined. The sequences were found to form two unique clusters not affiliated with any known bacterial species: cluster of unidentified sequences of free floating rumen fluid uncultured bacteria (FUB) and cluster of unidentified sequences of bacteria associated with rumen epithelium (AUB). A set of PCR primers targeting 16S rRNA of ruminal free uncultured bacteria and rumen epithelium adhering uncultured bacteria was designed based on these sequences. FUB primers were used for relative quantification of uncultured bacteria in ovine rumen samples. The effort to increase the population size of FUB group has been successful in sulfate reducing broth and culture media supplied with cellulose. (en)
Title	PCR detection of uncultured rumen bacteria PCR detection of uncultured rumen bacteria (en)
skos:prefLabel	PCR detection of uncultured rumen bacteria PCR detection of uncultured rumen bacteria (en)
skos:notation	RIV/67985904:_____/12:00379145!RIV13-GA0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA523/08/1091), P(GD525/08/H060), P(GPP503/10/P394), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Mrázek, Jakub Kopečný, Jan Fliegerová, Kateřina Štrosová, Lenka Rosero, Jamie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	158029
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/12:00379145
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DIVERSITY; METAGENOMICS (en)
http://linked.open.../riv/klicoveSlovo	DIVERSITY METAGENOMICS
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[387082BA22BB]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Synbiotics and plant extracts in human and animal nutrition Administration of bifidobacteria to young ruminants Impact of anaerobic fungi on biogas production
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Fliegerová, Kateřina Kopečný, Jan Mrázek, Jakub Štrosová, Lenka Rosero, Jamie
http://linked.open...ain/vavai/riv/wos	000305980400017
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0015-5632
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12223-012-0135-4

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