About: Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The present study was designed to extensively characterize cell lines derived from porcine blastocysts by several methodical approaches, including morphological observation, cytogenetic analysis, estimation of alkaline phosphatase activity and detection of specific marker expression at the mRNA/protein level. A comparison was made between the properties of cell lines isolated from in vivo- and in vitro-obtained blastocysts. Our results showed that 57.1% of the in vivo-obtained blastocysts attached to the feeder layer and that 33.3% of them started to grow in a monolayer. The percentage of attached in vitro-produced blastocysts was lower (24.6%), and only 6.9% of them started to grow. Outgrowths from the in vitro-produced blastocysts formed mainly trophectoderm or epithelial-like monolayer, whereas the in vivo-obtained blastocysts formed heterogeneous outgrowths that also contained cells with embryonic stem (ES)-like morphology. The present study was designed to extensively characterize cell lines derived from porcine blastocysts by several methodical approaches, including morphological observation, cytogenetic analysis, estimation of alkaline phosphatase activity and detection of specific marker expression at the mRNA/protein level. A comparison was made between the properties of cell lines isolated from in vivo- and in vitro-obtained blastocysts. Our results showed that 57.1% of the in vivo-obtained blastocysts attached to the feeder layer and that 33.3% of them started to grow in a monolayer. The percentage of attached in vitro-produced blastocysts was lower (24.6%), and only 6.9% of them started to grow. Outgrowths from the in vitro-produced blastocysts formed mainly trophectoderm or epithelial-like monolayer, whereas the in vivo-obtained blastocysts formed heterogeneous outgrowths that also contained cells with embryonic stem (ES)-like morphology. (en)
Title	Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo (en)
skos:prefLabel	Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo (en)
skos:notation	RIV/67985904:_____/11:00368327!RIV12-AV0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0538), P(QI101A166), Z(AV0Z50450515), Z(MZE0002701404)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Motlík, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	185693
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/11:00368327
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Blastocyst; In vivo and in vitro; Pig; Stem cells (en)
http://linked.open.../riv/klicoveSlovo	Pig Blastocyst In vivo and in vitro Stem cells
http://linked.open...odStatuVydavatele	JP - Japonsko
http://linked.open...ontrolniKodProRIV	[0986A69C112D]
http://linked.open...i/riv/nazevZdroje	Journal of Reproduction and Development
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Cell Therapy and Tissue Repair Biotechnology in pig husbandry and breeding
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Fulka, H. Kott, T. Krylov, V. Motlík, Jan Nováková, Z. Okada, K. Vacková, I.
http://linked.open...ain/vavai/riv/wos	000296597100006
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture Udržitelný rozvoj chovu hospodářských zvířat v evropském modelu multifunkčního zemědělství
issn	0916-8818
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1262/jrd.10-184H

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software