About: Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment

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About: Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation. Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation. (en)
Title	Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment (en)
skos:prefLabel	Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment (en)
skos:notation	RIV/67985904:_____/11:00367748!RIV12-AV0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0538), P(2B06130), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Juhás, Štefan Motlík, Jan Juhásová, Jana Strnádel, Ján Holubová, Monika Klíma, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	219038
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/11:00367748
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	miniature pig; mesenchymal stem cells; cell differentiation (en)
http://linked.open.../riv/klicoveSlovo	miniature pig cell differentiation mesenchymal stem cells
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[AECC19365437]
http://linked.open...i/riv/nazevZdroje	Physiological Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Cell Therapy and Tissue Repair Synthesis of new biomaterials and preparation of stem cell derived cells, and their aplications in for the treatment of diseases affecting human tissues derived from mesoderm: cartilage, bone, ligament and meniscus.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Juhás, Štefan Klíma, Jiří Motlík, Jan Juhásová, Jana Holubová, Monika Strnádel, Ján
http://linked.open...ain/vavai/riv/wos	000293138600017
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0862-8408
number of pages	13 (xsd:int)

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