About: Diversity of Anaerobic Fungi within Cow Manure Determined by ITS1 Analysis

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Description	The diversity of anaerobic fungi was evaluated in cow semiliquid manure obtained from input homogenizing tank of biogas plant. Among three sets of tested primers, the combination of fungal universal ITS1F and Neocallimastigales specific Neo QPCR Rev primers was selected and used for the construction of clone library. Eighty-four new complete internal transcribed spacers (ITS1) and partial 5.8S rDNA sequences generated within this study were analyzed by Bayesian inference and assigned to an existing order of the Neocallimastigales. Sixty-seven % of sequences were affiliated with Cyllamyces, 24 % with Piromyces, 7 % with Anaeromyces, only 2 % with Neocallimastix, and no sequences with Orpinomyces. According to Bayesian analysis the genus Caecomyces was polyphyletic and disappeared from the presented ITS-based phylogram. This study gave a first insight into the diversity of anaerobic fungi in cow manure, where the prevalence of fungi with bulbous morphology was indicated. The diversity of anaerobic fungi was evaluated in cow semiliquid manure obtained from input homogenizing tank of biogas plant. Among three sets of tested primers, the combination of fungal universal ITS1F and Neocallimastigales specific Neo QPCR Rev primers was selected and used for the construction of clone library. Eighty-four new complete internal transcribed spacers (ITS1) and partial 5.8S rDNA sequences generated within this study were analyzed by Bayesian inference and assigned to an existing order of the Neocallimastigales. Sixty-seven % of sequences were affiliated with Cyllamyces, 24 % with Piromyces, 7 % with Anaeromyces, only 2 % with Neocallimastix, and no sequences with Orpinomyces. According to Bayesian analysis the genus Caecomyces was polyphyletic and disappeared from the presented ITS-based phylogram. This study gave a first insight into the diversity of anaerobic fungi in cow manure, where the prevalence of fungi with bulbous morphology was indicated. (en)
Title	Diversity of Anaerobic Fungi within Cow Manure Determined by ITS1 Analysis Diversity of Anaerobic Fungi within Cow Manure Determined by ITS1 Analysis (en)
skos:prefLabel	Diversity of Anaerobic Fungi within Cow Manure Determined by ITS1 Analysis Diversity of Anaerobic Fungi within Cow Manure Determined by ITS1 Analysis (en)
skos:notation	RIV/67985904:_____/10:00347929!RIV11-GA0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GPP503/10/P394), P(QI92A286), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Mrázek, Jakub Fliegerová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	254802
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/10:00347929
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	GUT FUNGI; RUMEN; IDENTIFICATION (en)
http://linked.open.../riv/klicoveSlovo	IDENTIFICATION GUT FUNGI RUMEN
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[8DE37A7A8080]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EH
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Impact of anaerobic fungi on biogas production Enhancement of biogas production from phytomas by means of anaerobic fungi.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Fliegerová, Kateřina Mrázek, Jakub Zábranská, J. Hoffmann, K. Voigt, K.
http://linked.open...ain/vavai/riv/wos	000281277900004
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0015-5632
number of pages	6 (xsd:int)

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