About: AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes

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About: AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro. Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 mikroM SH-6, a specific The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro. Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 mikroM SH-6, a specific (en)
Title	AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes (en)
skos:prefLabel	AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes (en)
skos:notation	RIV/67985904:_____/09:00329884!RIV10-AV0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/06/1297), P(GA523/03/0857), P(GA524/07/1087), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kalous, Jaroslav Motlík, Jan Kubelka, Michal Šušor, Andrej Šolc, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	302268
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/09:00329884
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protein kinase; porcine oocyte; oocyte maturation (en)
http://linked.open.../riv/klicoveSlovo	oocyte maturation porcine oocyte protein kinase
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[665DBA6DB207]
http://linked.open...i/riv/nazevZdroje	Reproduction
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Regulation of translation initiation during meiotic maturation of pig oocytes by protein factors associated with mRNA 3'-end The changes in chromatin structure during meiotic maturation of pig oocytes: correlation to chromosomal aneuploidy The role of Akt kinase in the regulation of G2/M cell cycle transition and in the acquisition of developmental potency of oocytes
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	138
http://linked.open...iv/tvurceVysledku	Motlík, Jan Kalous, Jaroslav Kubelka, Michal Šušor, Andrej Šolc, Petr
http://linked.open...ain/vavai/riv/wos	000270806100004
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	1470-1626
number of pages	10 (xsd:int)

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