| Attributes | Values |
|---|
| rdf:type
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| Description
| - Přítomnost chitinu v buněčných stěnách je charakteristická pro mnoho hub včetně Chytridimycet. Tyto geny proto mají velký potenciál ve fylogenetickém využití, dosud však byly aplikovány hlavně u Askomycet, ale u Chytridiomycet nebyly použity. Aplikovali jsme proto primery Nag-forward a Nag-reverse na 5 anaerobních a 7 anaerobních chytridií. Experimenty prokázaly, že tyto primery jsou specifické pouze pro Emericella nudilans. Proto na základě konservativních sekvencí fungálních, protozálních a bakteriálních proteinů pro Nag1 z Genové Banky byl navržen set primerů pro universální amplifikaci genu nag1 u hub třídy Neocallimastigales. (cs)
- The common feature of all chytridiomycetous fungi, aerobic as well as anaerobic, is abundance of chitin in their cell wall. Genes encoding chitinases were widely used as phylogenetic markers in ascomycetes, but their utility was not tested for Chytridiomycetes. In this study we chose the gene encoding an enzyme involved in chitin degradation and energy metabolism, the b-(1, 4)-N-acetyl-glucosaminidase (Nag1). Primer pair Nag-fwd and Nag-rev was used to create PCR product from five strains of anaerobic and seven strains of aerobic chytrids. Blast search of sequenced amplicons however proved that these primers are specific only for fungus Emericella nidulans. Amino acid alignement of Nag1 proteins of fungal, protozoal and bacterial origin available in GenBank database was therefore performed in this work. Five amino acid regions were found to be conserved enough to serve as suitable domain for the design of set of primers for the universal amplification of the Nag1 gene in fungi.
- The common feature of all chytridiomycetous fungi, aerobic as well as anaerobic, is abundance of chitin in their cell wall. Genes encoding chitinases were widely used as phylogenetic markers in ascomycetes, but their utility was not tested for Chytridiomycetes. In this study we chose the gene encoding an enzyme involved in chitin degradation and energy metabolism, the b-(1, 4)-N-acetyl-glucosaminidase (Nag1). Primer pair Nag-fwd and Nag-rev was used to create PCR product from five strains of anaerobic and seven strains of aerobic chytrids. Blast search of sequenced amplicons however proved that these primers are specific only for fungus Emericella nidulans. Amino acid alignement of Nag1 proteins of fungal, protozoal and bacterial origin available in GenBank database was therefore performed in this work. Five amino acid regions were found to be conserved enough to serve as suitable domain for the design of set of primers for the universal amplification of the Nag1 gene in fungi. (en)
|
| Title
| - The design of oligonucleotide primers for the universal amplification of the N-acetylglucosaminidase gene (nag1) in Chytridiomycetes with emphasis on the anaerobic Neocallimastigales
- Návrh primerů pro universální amplifikaci N-acetylglukozaminidázového genu (nag1) pro Chytridiomycety s důrazem na anaerobní Neocallimastigales (cs)
- The design of oligonucleotide primers for the universal amplification of the N-acetylglucosaminidase gene (nag1) in Chytridiomycetes with emphasis on the anaerobic Neocallimastigales (en)
|
| skos:prefLabel
| - The design of oligonucleotide primers for the universal amplification of the N-acetylglucosaminidase gene (nag1) in Chytridiomycetes with emphasis on the anaerobic Neocallimastigales
- Návrh primerů pro universální amplifikaci N-acetylglukozaminidázového genu (nag1) pro Chytridiomycety s důrazem na anaerobní Neocallimastigales (cs)
- The design of oligonucleotide primers for the universal amplification of the N-acetylglucosaminidase gene (nag1) in Chytridiomycetes with emphasis on the anaerobic Neocallimastigales (en)
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| skos:notation
| - RIV/67985904:_____/08:00324753!RIV09-AV0-67985904
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA523/05/2555), Z(AV0Z50450515)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/67985904:_____/08:00324753
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - chytridiomycetous fungi (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Fliegerová, Kateřina
- Mrázek, Jakub
- Hoffmann, K.
- Voigt, K.
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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