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| Description
| - To determine the organization of transferrin (TF) locus in the Salmo trutta genome, partial DNA and cDNA sequencing, fluorescent in situ hybridization (FISH) and Salmo salar BAC analysis were performed. The real-time PCR data showed that each of the two TF types (TF1 and TF2) existed in one copy only and that the transcription of TF2 was considerably lower compared with TF1. Using FISH, hybridization signals were observed on two medium-sized acrocentric chromosomes of S. trutta karyotype. A TF type-specific PCR followed by a restriction analysis revealed the presence of two TF loci in the majority of analysed BAC clones. It was concluded that the TF gene is duplicated in the genome of S. trutta, and that the two TF loci are located adjacent to one another on the same chromosome. The differing transcription levels of TF1 and TF2 appear to depend on the corresponding promoter activity, which at least for TF2 seems to vary between different Salmo congeners.
- To determine the organization of transferrin (TF) locus in the Salmo trutta genome, partial DNA and cDNA sequencing, fluorescent in situ hybridization (FISH) and Salmo salar BAC analysis were performed. The real-time PCR data showed that each of the two TF types (TF1 and TF2) existed in one copy only and that the transcription of TF2 was considerably lower compared with TF1. Using FISH, hybridization signals were observed on two medium-sized acrocentric chromosomes of S. trutta karyotype. A TF type-specific PCR followed by a restriction analysis revealed the presence of two TF loci in the majority of analysed BAC clones. It was concluded that the TF gene is duplicated in the genome of S. trutta, and that the two TF loci are located adjacent to one another on the same chromosome. The differing transcription levels of TF1 and TF2 appear to depend on the corresponding promoter activity, which at least for TF2 seems to vary between different Salmo congeners. (en)
- Organizace genu pro transferin v genomu Salmu trutta byla studpvána pomocí DNA a cDNA sekvenování, in situ hybridizací (FISH) a BAC knihovnami z genomu Salmo salar. RT-PCR ukázala, že každá z obou typu TF existuje jen v jedné kopii a transkripční aktivita druhé je daleko nižší. Fyzická lokalizace TF lokusů pomocí FISH zjistila jejich místo v pericentromerické oblasti ne jednom páru středně velkých akrocentrických chromozómů. Analýza ukázala, ze TF lokus je duplikován a obě kopie leží tandemově za sebou na tom samém chromozómu. (cs)
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| Title
| - Evidence for two transferrin loci in the Salmo trutta genome
- Evidence for two transferrin loci in the Salmo trutta genome (en)
- Důkaz dvou lokusů pro transferin v genomu Salmo trutta (cs)
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| skos:prefLabel
| - Evidence for two transferrin loci in the Salmo trutta genome
- Evidence for two transferrin loci in the Salmo trutta genome (en)
- Důkaz dvou lokusů pro transferin v genomu Salmo trutta (cs)
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| skos:notation
| - RIV/67985904:_____/08:00314274!RIV09-AV0-67985904
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/67985904:_____/08:00314274
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - chromosomes; fluorescent in situ hybridization; real-time PCR (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Ráb, Petr
- Dovc, P.
- Erjavec-Škerget, A.
- Kokalj-Vokač, N.
- Marič, S.
- Rozman, T.
- Snoj, A.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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