About: Characterization of chitinases of polycentric anaerobic rumen fungi

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Chitinolytický systém anaerobních polycentrických bachorových hub rodu Orpinomyces a Anaeromyces byl studován ve třech frakcích- extracelulární, cytosolické frakce a frakce z buněčných stěn. Endochitinasa představovala dominantní enzym s nejvyšší aktivitou v cytosolické frakci. Endochitinasa u obou rodů byla stabilní v rozmezí pH 4,5 až 7 s optimálním pH 6,5. Endochitinasa u Orpinomyces byla stabilní do 50°C s optimální teplotou 50°C. Endochitinasa z Anaeromyces byla stabilní do 40°C s optimální teplotou 40°C. Nejvhodnějším substrátem pro endochitinasu u obou rodů byl chitin izolovaný z houbových buněčných stěn. Enzymová aktivita obou endochitinas byla inhibována iontem Hg2+ a aktivována iontem Mg2+. Obě endochitinasy byli inhibovány 10 mM SDS a aktivovány iodoacetamidem. (cs) Chitinolytic system of anaerobic polycentric rumen fungi of genera Orpinomyces and Anaeromyces was studied in three crude enzyme fractions- extracellular, cytosolic and cell wall fraction. Endochitinase was found as a dominant enzyme with highest activity in cytosolic fraction. Endochitinases of both genera were stable from pH 4.5 to 7 with optimal pH 6.5. Orpinomyces endochitinase was stable up to 50 0C with optimal temperature 50 0C. Anaeromyces endochitinase was stable up to 40 0C with optimal temperature also 40 0C. The most suitable substrate for both endochitinases was fungal cell wall chitin. Enzyme activities were inhibited by Hg2+ ions and activated by Mg2+ ions. Both endochitinases were inhibited by 10 mM SDS and activated by iodacetamide. Chitinolytic system of anaerobic polycentric rumen fungi of genera Orpinomyces and Anaeromyces was studied in three crude enzyme fractions- extracellular, cytosolic and cell wall fraction. Endochitinase was found as a dominant enzyme with highest activity in cytosolic fraction. Endochitinases of both genera were stable from pH 4.5 to 7 with optimal pH 6.5. Orpinomyces endochitinase was stable up to 50 0C with optimal temperature 50 0C. Anaeromyces endochitinase was stable up to 40 0C with optimal temperature also 40 0C. The most suitable substrate for both endochitinases was fungal cell wall chitin. Enzyme activities were inhibited by Hg2+ ions and activated by Mg2+ ions. Both endochitinases were inhibited by 10 mM SDS and activated by iodacetamide. (en)
Title	Characterization of chitinases of polycentric anaerobic rumen fungi Characterization of chitinases of polycentric anaerobic rumen fungi (en) Charakterizace chitinas polycentrických anaerobních bachorových hub (cs)
skos:prefLabel	Characterization of chitinases of polycentric anaerobic rumen fungi Characterization of chitinases of polycentric anaerobic rumen fungi (en) Charakterizace chitinas polycentrických anaerobních bachorových hub (cs)
skos:notation	RIV/67985904:_____/08:00312643!RIV09-AV0-67985904
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Novotná, Zuzana Šimůnek, Jiří Fliegerová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	359578
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/08:00312643
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	endochitinase; anaerobic; rumen fungi (en)
http://linked.open.../riv/klicoveSlovo	anaerobic endochitinase rumen fungi
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[1C286AB424B3]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Fliegerová, Kateřina Šimůnek, Jiří Novotná, Zuzana
http://linked.open...ain/vavai/riv/wos	000257941200014
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0015-5632
number of pages	5 (xsd:int)

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