About: Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Kinex antibody microarray analyses was used to investigate the regulation of 188 protein kinases, 24 protein phosphatases, and 170 other regulatory proteins during meiotic maturation of immature germinal vesicle (GV+) pig oocytes to maturing oocytes that had completed meiosis I (MI), and fully mature oocytes arrested at metaphase of meiosis II (MII). Western blotting analyses confirmed altered expression levels of Bub1A, IRAK4, MST2, PP4C, and Rsk2, and the phosphorylation site changes in the kinases Erk5 (T218 + Y220), FAK(S722), GSK3-beta (Y216), MEK1 (S217 + S221) and PKR1 (T451), and nucleophosmin/B23 (S4) during pig oocyte maturation. Kinex antibody microarray analyses was used to investigate the regulation of 188 protein kinases, 24 protein phosphatases, and 170 other regulatory proteins during meiotic maturation of immature germinal vesicle (GV+) pig oocytes to maturing oocytes that had completed meiosis I (MI), and fully mature oocytes arrested at metaphase of meiosis II (MII). Western blotting analyses confirmed altered expression levels of Bub1A, IRAK4, MST2, PP4C, and Rsk2, and the phosphorylation site changes in the kinases Erk5 (T218 + Y220), FAK(S722), GSK3-beta (Y216), MEK1 (S217 + S221) and PKR1 (T451), and nucleophosmin/B23 (S4) during pig oocyte maturation. (en) Kinex protilátkové mikročipy byly použity ke sledování regulace 188 protein kináz, 24 protein fosfatáz a 170 další regulačních proteinů v průběhu zrání prasečích oocytů z GV stadia do metafáze MII. Regulace vybraných proteinů byla paralelně sledována u oocytů krav, žab a mořské hvězdice. Specifický imunoblot potvrdil změny hladin či fosforylace u Bub1A, IRAK4, MST2, PP4C, and Rsk2, Erk5 (T218 + Y220), FAK(S722), GSK3-beta (Y216), MEK1 (S217 + S221) and PKR1 (T451), and nucleophosmin/B23 (S4). (cs)
Title	Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation (en) Analýza signálních proteinů a fosforylace v průběhu zrání prasečích oocytů s využitím protilátkových mikročipů (cs)
skos:prefLabel	Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation (en) Analýza signálních proteinů a fosforylace v průběhu zrání prasečích oocytů s využitím protilátkových mikročipů (cs)
skos:notation	RIV/67985904:_____/08:00310174!RIV09-AV0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/06/1297), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Kovářová, Hana Jelínková, Lucie Kubelka, Michal Pavlok, Antonín Šušor, Andrej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	356400
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/08:00310174
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antibody microarray; pig; frog (en)
http://linked.open.../riv/klicoveSlovo	frog pig antibody microarray
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[05F61849DBC8]
http://linked.open...i/riv/nazevZdroje	Journal of Proteome Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	The changes in chromatin structure during meiotic maturation of pig oocytes: correlation to chromosomal aneuploidy
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Shi, X. Zhang, H. Kovářová, Hana Kubelka, Michal Pavlok, Antonín Šušor, Andrej Jelínková, Lucie Pelech, S.
http://linked.open...ain/vavai/riv/wos	000257449500027
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	1535-3893
number of pages	12 (xsd:int)

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