| Attributes | Values |
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| rdf:type
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| Description
| - Savčí zuby se zakládají embryonálně jako epitelio-mezenchymální orgány. Primární sklovinný uzel představuje signální centrum morfogeneze dentice. Tento útvar podstupuje apoptózu po vytvoření zubního zvonku a aktivace kaspázy 3 představuje centrální krok v intracelulární apoptotické mašinérii. Prokaspáza 3 a kaspáza 3 byly lokalizovány v primárním sklovinném uzlu s využitím imunohistochemie za použití různých fixačních postupů. Výsledky byly hodnoceny po vizualizaci sledovaných molekul peroxidázou zprostředkovanou barevnou reakcí. Apoptóza byla hodnocena s využitím morfologických kritérií a TUNEL testu. Prokaspáza byla nalezena jak v epiteliální, tak mezenchymální části zubních základů, aktivní kaspáza 3 především v primárním sklovinném uzlu a její distribuce korelovala s výskytem apoptózy. Časoprostorové rozložení aktivní kaspázy 3 u hraboše a laboratorní myši bylo obdobné. (cs)
- The mammalian teeth develop during embryogenesis as epithelio-mesenchymal organs. The primary enamel knot is considered as a signalling centre in tooth morphogenesis. After tooth bell formation, this epithelial structure undergoes apoptosis. Activation of caspase 3 represents a central step in the intracellular death machinery. Procaspase 3 and caspase 3 molecules were localized in the primary enamel knot of the field vole using immunohistochemistry. Different fixation procedures in cryopreserved and paraffin embedded tissues and detection systems based on peroxidase and alkaline phosphatase mediated colour reactions were applied and evaluated. Apoptosis was detected using morphological criteria and TUNEL assay. Procaspase 3 was found in both epithelial and mesenchymal part of the tooth germ. Active caspase 3 was localized particularly in the primary enamel knot, its distribution correlated with dental apoptosis and showed a similar pattern in the field vole as in the mouse.
- The mammalian teeth develop during embryogenesis as epithelio-mesenchymal organs. The primary enamel knot is considered as a signalling centre in tooth morphogenesis. After tooth bell formation, this epithelial structure undergoes apoptosis. Activation of caspase 3 represents a central step in the intracellular death machinery. Procaspase 3 and caspase 3 molecules were localized in the primary enamel knot of the field vole using immunohistochemistry. Different fixation procedures in cryopreserved and paraffin embedded tissues and detection systems based on peroxidase and alkaline phosphatase mediated colour reactions were applied and evaluated. Apoptosis was detected using morphological criteria and TUNEL assay. Procaspase 3 was found in both epithelial and mesenchymal part of the tooth germ. Active caspase 3 was localized particularly in the primary enamel knot, its distribution correlated with dental apoptosis and showed a similar pattern in the field vole as in the mouse. (en)
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| Title
| - Caspase 3 activation in the primary enamel knot of developing molar tooth
- Aktivace kaspázy 3 v primárním sklovinném uzlu při vývoji moláru (cs)
- Caspase 3 activation in the primary enamel knot of developing molar tooth (en)
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| skos:prefLabel
| - Caspase 3 activation in the primary enamel knot of developing molar tooth
- Aktivace kaspázy 3 v primárním sklovinném uzlu při vývoji moláru (cs)
- Caspase 3 activation in the primary enamel knot of developing molar tooth (en)
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| skos:notation
| - RIV/67985904:_____/06:00040992!RIV07-AV0-67985904
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA304/04/0101), P(KJB500450503), P(OC B23.001), Z(AV0Z50450515)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/67985904:_____/06:00040992
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - apoptosis; caspase 3; primary enamel knot (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Míšek, Ivan
- Matalová, Eva
- Kovářů, František
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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