About: Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP

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About: Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	PCR produkty z malé 18S a velké 28S ribozomální podjednotky a ITS spaceru byly podrobeny restrikční analýze za použití restrikčních enzymů AluI, HinfI, DraI. Rody Orpinomyses a Anaeromyces se nelišily v RFLP profilu pro malou ribozomální podjednotku 18S, avšak vykazovaly rozdílné restrikční profily při štěpení fragmentů podjednotky 28S a ITS fragmentu. (cs) The suitability of restriction fragment lenght polymorphism (RFLP) analysis of the ribosomal DNA cluster for discriminating two genera of anaerobic polycentric fungi, Orcinomyces and Anaeromyces, was determined. Three PCR-amplified DNA fragments - nuclear small subunit 18S and the nuclear large subunit S28 and internal transcribed spacer (ITS)-were restricted with endonucleases AluI, HinfI, DraI. Although the SSU DNA fragment could be restricted successfully by all four enzymes, no differences were observed between restriction patterns of Orpinomyces and Anaeromyces. Genus-specific RFLP patterns were determined for Orpinomyces and Anaeromyces genera; the results showed that the PCR-RFLP analysis of rDNA offers an easy and rapid tool for differentiation of two polycentric genera of anaerobic fungi, which could be hardly separated on the basis of morphology. The suitability of restriction fragment lenght polymorphism (RFLP) analysis of the ribosomal DNA cluster for discriminating two genera of anaerobic polycentric fungi, Orcinomyces and Anaeromyces, was determined. Three PCR-amplified DNA fragments - nuclear small subunit 18S and the nuclear large subunit S28 and internal transcribed spacer (ITS)-were restricted with endonucleases AluI, HinfI, DraI. Although the SSU DNA fragment could be restricted successfully by all four enzymes, no differences were observed between restriction patterns of Orpinomyces and Anaeromyces. Genus-specific RFLP patterns were determined for Orpinomyces and Anaeromyces genera; the results showed that the PCR-RFLP analysis of rDNA offers an easy and rapid tool for differentiation of two polycentric genera of anaerobic fungi, which could be hardly separated on the basis of morphology. (en)
Title	Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP (en) Diferenciace anaerobních polycentrických hub na základe rDNA PCR-RFLP analýzy (cs)
skos:prefLabel	Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP (en) Diferenciace anaerobních polycentrických hub na základe rDNA PCR-RFLP analýzy (cs)
skos:notation	RIV/67985904:_____/06:00040840!RIV07-AV0-67985904
http://linked.open.../vavai/riv/strany	273;277
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA523/05/2555), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Mrázek, Jakub Fliegerová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	471659
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/06:00040840
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	anaerobic polycentric fungi (en)
http://linked.open.../riv/klicoveSlovo	anaerobic polycentric fungi
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[4F584A2AA9CE]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Deep phylogeny of anaerobic fungi
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Fliegerová, Kateřina Mrázek, Jakub Voigt, K.
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0015-5632
number of pages	6 (xsd:int)

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