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| Description
| - Aurora-B kinase is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we have examined expression and phosphorylation (Thr-232) of Aurora B kinase during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser-10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, while Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 mM Butyrolactone I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10mg/ml), inhibitor of proteosynthesis, results in a block of oocytes in the germin...
- Aurora-B kinase is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we have examined expression and phosphorylation (Thr-232) of Aurora B kinase during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser-10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, while Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 mM Butyrolactone I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10mg/ml), inhibitor of proteosynthesis, results in a block of oocytes in the germin... (en)
- Aurora B kináza je tzv. chromosomální „passenger“ protein, který je nezbytný pro řadu procesů probíhajíchích během mitózy. Jeho aktivita je regulována spojením s dalšími „passenger“ proteiny, INCENP a Survivin, a jeho fosforylací na Thr-232. V této práci, byla studována exprese a fosforylace (Thr-232) Aurory B kinázy během meiotického zrání prasečích oocytů spolu s fosforylací histonu H3 a v korelaci s kondenzací chromosomů. Ukázalo se, že fosforylace histonu H3 na Ser-10, ale ne na Ser-28, koreluje s postupnou kondenzací chromosomů během zrání oocytů; fosforylace na Ser 10 začíná v okolí doby rozpadu jaderné membrány, s maximální aktivitou v metafázi I, zatímco fosforylace na Ser-28 se během zrání oocytů výrazně nemění. Po ošetření oocytů 50 mM Butyrolactonem I (BL-I), inhibitor cyklin dependentních kináz, či cykloheximidem ( 10mg/ml) – inhibitor proteosyntézy, dochází k zastavení oocytu ve fázi tzv. zárodečného váčku (germinal vesicle – GV), ve které zůstává neporušena jaderná membrána, nicm... (cs)
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| Title
| - Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes
- Pro kondenzaci chromosomů během meotického zrání prasečích oocytů není nezbytná ani aktivita Aurory B ani fosforylace histonu H3 (cs)
- Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes (en)
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| skos:prefLabel
| - Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes
- Pro kondenzaci chromosomů během meotického zrání prasečích oocytů není nezbytná ani aktivita Aurory B ani fosforylace histonu H3 (cs)
- Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes (en)
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| skos:notation
| - RIV/67985904:_____/06:00040831!RIV07-AV0-67985904
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA204/03/0816), Z(AV0Z50450515)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/67985904:_____/06:00040831
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - gamete biology; kinases; meiosis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kubelka, Michal
- Jelínková, Lucie
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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