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| rdf:type
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| Description
| - Cílem této studie bylo srovnat expresy vybraných genů u bovinních embryí vyvíjejících se z oocytů s rozdílnou vývojovou kompetencí. Čtyři populace oocytů byly izolovány, buď z malých (2-5 mm) nebo středních (6-10 mm) folikulů, ve stadiu růstu/stagnace (G/S), nebo dominance/regrese (D/R) první folikulární vlny. Oocyty byly dozrávány, oplozeny a kultivovány podle standardního protokolu do stadií D7 nebo D8 blastocyst. Poly(A)+ mRNA byla izolována ze skupin oocytů a exprese bax α (Bax), connexinu 43 (Cx43) a connexinu 31 (Cx31) byla měřena pomocí real-time RT-PCR. Procento dělení bylo signifikantně vyšší u oocytů, izolovaných ze středních a malých folikulů (p Ł 0,05 a p Ł 0,01) v G/S než v D/R stadiu. Relativní obsah Bax a Cx31 se výrazně nelišil u D7 a D8 blastocyst, které se vyvinuly z oocytů izolovaných ze středních a malých folikulů v G/S stadiu než těmi v D/R stadiu. Avšak relativní obsah Cx43 transkriptu byl významně vyšší u D8 blastocyst, které se vyvinuly z oocytů izolovaných ze středních a ... (cs)
- The aim of this study was to compare the expression of selected genes in bovine embryos developed from oocytes with different meiotic competence collected from different-sized follicles in two stages of follicular development. Oocyte subpopulations were collected from the ovaries of individual donors, separately from small and medium follicles, either in the growth/stagnation (G/S) or dominance/regression (D/R) stage. They were matured, fertilized and cultured to D7 or D8 blastocysts by the standard protocol. Poly (A)+ mRNA was extracted from pooled blastocysts and the expression of bax-α (Bax), connexin 43 (Cx 43) and connexin 31 (Cx 31) was estimated using real-time RT-PCR. The cleavage rates were significantly higher in oocytes collected from both medium and small follicles, (pŁ 0.05 and pŁ 0.01, respectively) in the G/S than in the D/R stage. There were no significant differences in the D7 blastocyst rates between oocytes from both medium and small follicles in the G/S or D/R stage. But the D8...
- The aim of this study was to compare the expression of selected genes in bovine embryos developed from oocytes with different meiotic competence collected from different-sized follicles in two stages of follicular development. Oocyte subpopulations were collected from the ovaries of individual donors, separately from small and medium follicles, either in the growth/stagnation (G/S) or dominance/regression (D/R) stage. They were matured, fertilized and cultured to D7 or D8 blastocysts by the standard protocol. Poly (A)+ mRNA was extracted from pooled blastocysts and the expression of bax-α (Bax), connexin 43 (Cx 43) and connexin 31 (Cx 31) was estimated using real-time RT-PCR. The cleavage rates were significantly higher in oocytes collected from both medium and small follicles, (pŁ 0.05 and pŁ 0.01, respectively) in the G/S than in the D/R stage. There were no significant differences in the D7 blastocyst rates between oocytes from both medium and small follicles in the G/S or D/R stage. But the D8... (en)
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| Title
| - Gene expression in bovine embryos derived from oocytes with different developmental competence collected at the defined follicular developmental stage
- Gene expression in bovine embryos derived from oocytes with different developmental competence collected at the defined follicular developmental stage (en)
- Exprese genů u embryí skotu získaných z oocytů s rozdílnou vývojovou kompetencí, izolovaných v definovaném stadiu folikulárního vývoje (cs)
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| skos:prefLabel
| - Gene expression in bovine embryos derived from oocytes with different developmental competence collected at the defined follicular developmental stage
- Gene expression in bovine embryos derived from oocytes with different developmental competence collected at the defined follicular developmental stage (en)
- Exprese genů u embryí skotu získaných z oocytů s rozdílnou vývojovou kompetencí, izolovaných v definovaném stadiu folikulárního vývoje (cs)
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| skos:notation
| - RIV/67985904:_____/06:00040790!RIV07-AV0-67985904
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA524/02/1135), Z(AV0Z50450515), Z(MZE0002716201)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/67985904:_____/06:00040790
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - cattle; follicular development; oocyte development (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Horáková, J.
- Němcová, Lucie
- Hanzalová, K.
- Kaňka, Jiří
- Machatková, M.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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