About: Occurrence of restriction–modification systems in ruminal butyrate-producing bacteria

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About: Occurrence of restriction–modification systems in ruminal butyrate-producing bacteria Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Thirty-five strains of ruminal bacteria belonging to the former Butyrivibrio fibrosolvens species were screened for the presence of site-specific restriction endonuclease and modification methyltransferase activities. The recognition sequences and otimal reaction conditions for seven of them were determined. Five enzymes were found to be isoschizomers of type II endonucleases, one was type IIS (FokI) and two remained unknown. The otimal reaction buffer was found to be a low ionic strength buffer and all enzymes possessed sufficient activity at 39°C. The presence of DNA modification among all strains was also determined. Thirty-five strains of ruminal bacteria belonging to the former Butyrivibrio fibrosolvens species were screened for the presence of site-specific restriction endonuclease and modification methyltransferase activities. The recognition sequences and otimal reaction conditions for seven of them were determined. Five enzymes were found to be isoschizomers of type II endonucleases, one was type IIS (FokI) and two remained unknown. The otimal reaction buffer was found to be a low ionic strength buffer and all enzymes possessed sufficient activity at 39°C. The presence of DNA modification among all strains was also determined. (en) Byl popsán restrikčně modifikační systém u anaerobních bakterií rodů Butyrivibrio a Pseudobutyrivibrio. Většinou se jednalo o endonukleasy II typu s aktivitou EcoRV, NsiI, AseI, a SauI. Byla zjištěna i endonukleasa IIS typu. Dvě aktivity zatím nejsou ještě popsány. Všechny izoláty se specifickými restriktasami obsahovaly i DNA modifikační metyltransferasy. (cs)
Title	Occurrence of restriction–modification systems in ruminal butyrate-producing bacteria Výskyt restrikčně-modifikačního systému u bachorových bakterií produkujících kyselinu máselnou (cs) Occurrence of restriction–modification systems in ruminal butyrate-producing bacteria (en)
skos:prefLabel	Occurrence of restriction–modification systems in ruminal butyrate-producing bacteria Výskyt restrikčně-modifikačního systému u bachorových bakterií produkujících kyselinu máselnou (cs) Occurrence of restriction–modification systems in ruminal butyrate-producing bacteria (en)
skos:notation	RIV/67985904:_____/05:00027362!RIV06-AV0-67985904
http://linked.open.../vavai/riv/strany	280;284
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IBS5045112), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Mrázek, Jakub Kopečný, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	533940
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/05:00027362
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	restriction endonuclease (en)
http://linked.open.../riv/klicoveSlovo	restriction endonuclease
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[A874B2F8B3EE]
http://linked.open...i/riv/nazevZdroje	Anaerobe
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Transformation system for rumen anaerobic bacterium Butyrivibrio fibrisolvens
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Kopečný, Jan Mrázek, Jakub Pristaš, P. Piknová, M.
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	1075-9964
number of pages	5 (xsd:int)

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