Attributes | Values |
---|
rdf:type
| |
Description
| - Cílem studie bylo pro ochranu sumce velkého ex situ vypracovat techniku kryokonzervace spermatu. Úspěšnost byla posuzována po rozmrazení dávek na základě motility, rychlosti pohybu, % živých spermií a líhnivosti. Nejlepší líhnivost 82–86% byla dosažena u dávek uchovávaných 5 h před mražením v imobilizačním roztoku a mražených v 8, 10 a 12% Me2SO nebo ve směsi 5% Me2SO a 5% propandiolu. Výsledky se nelišily od kontroly s použitím čerstvého spermatu. Procento živých spermií v rozmražených dávkách nekorelovalo s líhnivostí nebo motilitou spermií, ale negativně korelovalo s rychlostí pohybu spermií (r = ?0.47, P = 0.05). Motilita spermií v rozmrazených dávkách se pohybovala od 8 do 62% u dávek uchovávaných 5 h před mražením v imobilizačním roztoku; průměrná hodnota kontroly byla 96%. Motilita spermií v rozmrazených dávkách významně korelovala s líhnivostí (r = 0.76, P = 0.0002), ne však s % živých spermií (r = 0.16, P = 0.52) ani s rychlostí pohybu (r = 0.07, P = 0.79). Rychlost pohybu... (cs)
- The aim of this study was to elaborate cryopreservation methods for ex situ conservation of European catfish. The success of sperm cryopreservation was evaluated by post-thaw sperm motility and velocity, percentage of live spermatozoa and fertility (hatching rates) using frozen/thawed sperm. The best hatching rates of 82–86% were obtained with sperm stored for 5 h before freezing in immobilizing solution and frozen with Me2SO in concentrations of 8, 10, and 12%, or with a mixture of 5% Me2SO and 5% propandiole. These results did not significantly differ from the fresh sperm control sample. The percentage of live spermatozoa in frozen/thawed sperm did not correlate with hatching rate or motility of spermatozoa, but was negatively correlated with velocity of spermatozoa (r = ?0.47, P = 0.05). The percentage motility in frozen/thawed sperm ranged from 8 to 62%, when sperm was stored in immobilizing solution 5 h before freezing. The average value in the fresh sperm (control) was 96%. Th...
- The aim of this study was to elaborate cryopreservation methods for ex situ conservation of European catfish. The success of sperm cryopreservation was evaluated by post-thaw sperm motility and velocity, percentage of live spermatozoa and fertility (hatching rates) using frozen/thawed sperm. The best hatching rates of 82–86% were obtained with sperm stored for 5 h before freezing in immobilizing solution and frozen with Me2SO in concentrations of 8, 10, and 12%, or with a mixture of 5% Me2SO and 5% propandiole. These results did not significantly differ from the fresh sperm control sample. The percentage of live spermatozoa in frozen/thawed sperm did not correlate with hatching rate or motility of spermatozoa, but was negatively correlated with velocity of spermatozoa (r = ?0.47, P = 0.05). The percentage motility in frozen/thawed sperm ranged from 8 to 62%, when sperm was stored in immobilizing solution 5 h before freezing. The average value in the fresh sperm (control) was 96%. Th... (en)
|
Title
| - Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability and hatching success of embryos
- Kryokonzervace spermatu sumce velkého, Silurus glanis: Motilita a životnost spermatu a líhnivost embryí (cs)
- Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability and hatching success of embryos (en)
|
skos:prefLabel
| - Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability and hatching success of embryos
- Kryokonzervace spermatu sumce velkého, Silurus glanis: Motilita a životnost spermatu a líhnivost embryí (cs)
- Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability and hatching success of embryos (en)
|
skos:notation
| - RIV/67985904:_____/05:00024865!RIV06-AV0-67985904
|
http://linked.open.../vavai/riv/strany
| |
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(GA524/03/0178), P(IBS5045314), Z(AV0Z50450515), Z(MSM6007665809)
|
http://linked.open...iv/cisloPeriodika
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/67985904:_____/05:00024865
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - fish; sperm; aquaculture (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/nazevZdroje
| |
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...v/svazekPeriodika
| |
http://linked.open...iv/tvurceVysledku
| - Flajšhans, Martin
- Gela, David
- Linhart, Otomar
- Rodina, Marek
- Kocour, Martin
|
http://linked.open...n/vavai/riv/zamer
| |
issn
| |
number of pages
| |