About: Development of competitive PCR for detection of Butyrivibrio fibrosolvens isolates.

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of competitive PCR for detection of Butyrivibrio fibrosolvens isolates. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Competitive PCR method was developed for the detection and enumeration of Butyrivibrio fibriosolvens. Sequences of 16S rDNA were obtained from our isolates and were distinguished into nine different groups of butyrivibria. Specific primers for two distinct groups were designed with the help of BioEdit program. These primers were tested with DNA of 20 strains of ruminal B.fibrisolvens isolated. Annealing temperature 58 C showed a little specificity but a better selectivity was found after raising it p to 65 C. A group 1 competitive fragment og 16S rDNA of different lenght was constructed using restriction cutting with Mspl followed by ligation., the size of the resulting fragment was cut down by 75 bp. The fragment worked in the presence of the original 16S rDNA fragment of B.fibrisolvens JK 609. Competitive PCR method was developed for the detection and enumeration of Butyrivibrio fibriosolvens. Sequences of 16S rDNA were obtained from our isolates and were distinguished into nine different groups of butyrivibria. Specific primers for two distinct groups were designed with the help of BioEdit program. These primers were tested with DNA of 20 strains of ruminal B.fibrisolvens isolated. Annealing temperature 58 C showed a little specificity but a better selectivity was found after raising it p to 65 C. A group 1 competitive fragment og 16S rDNA of different lenght was constructed using restriction cutting with Mspl followed by ligation., the size of the resulting fragment was cut down by 75 bp. The fragment worked in the presence of the original 16S rDNA fragment of B.fibrisolvens JK 609. (en)
Title	Development of competitive PCR for detection of Butyrivibrio fibrosolvens isolates. Development of competitive PCR for detection of Butyrivibrio fibrosolvens isolates. (en)
skos:prefLabel	Development of competitive PCR for detection of Butyrivibrio fibrosolvens isolates. Development of competitive PCR for detection of Butyrivibrio fibrosolvens isolates. (en)
skos:notation	RIV/67985904:_____/01:21010002!RIV/2003/AV0/A21003/N
http://linked.open.../vavai/riv/strany	63;65
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KSK5052113), Z(AV0Z5045916)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Mrázek, Jakub Kopečný, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	677509
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/01:21010002
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	butyrivibrio fibrosolvens; - strains (en)
http://linked.open.../riv/klicoveSlovo	butyrivibrio fibrosolvens - strains
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[F36C90BCCF33]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure, expression and interaction of the genome
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	46
http://linked.open...iv/tvurceVysledku	Kopečný, Jan Mrázek, Jakub
http://linked.open...n/vavai/riv/zamer	Základní výzkum genetických struktur, fyziologických funkcí a vývojové biologie obratlovců se zaměřením na biomedicínsky, hospodářsky i jinak významné druhy
issn	0015-5632
number of pages	2 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software