About: Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases.

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About: Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohemine, either alone or in combination with ionomycin, is able to activate young matured bovine oocytes. Furthermore, the effect of bohemine on the patterns of DNA synthesis after pronuclear formation as well as changes in his tone H1 kinase and MAP kinase activities during the process of activation were studied. Our results with bohemine show that the specific inhibition of CDKs in metaphase II bovine oocytes induces parthenogenetic activation in a dose-dependent manner (25, 50, and 100 mu M, respectively), either alone (3%, 30%, and 50%) or in combination with ionomycin (30%, 70%, and 87.5%). A single pronucleus and extrusion of the second polar body was observed (97%) when Ca2+ influx was Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohemine, either alone or in combination with ionomycin, is able to activate young matured bovine oocytes. Furthermore, the effect of bohemine on the patterns of DNA synthesis after pronuclear formation as well as changes in his tone H1 kinase and MAP kinase activities during the process of activation were studied. Our results with bohemine show that the specific inhibition of CDKs in metaphase II bovine oocytes induces parthenogenetic activation in a dose-dependent manner (25, 50, and 100 mu M, respectively), either alone (3%, 30%, and 50%) or in combination with ionomycin (30%, 70%, and 87.5%). A single pronucleus and extrusion of the second polar body was observed (97%) when Ca2+ influx was (en)
Title	Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases. Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases. (en)
skos:prefLabel	Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases. Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases. (en)
skos:notation	RIV/67985904:_____/00:21003029!RIV/2003/AV0/A21003/N
http://linked.open.../vavai/riv/strany	422;432
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KSK2038602), Z(AV0Z5045916)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Motlík, Jan Kubelka, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	703878
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/00:21003029
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	N/A (en)
http://linked.open.../riv/klicoveSlovo	N/A
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E0D2AAE60364]
http://linked.open...i/riv/nazevZdroje	Molecular Reproduction and Development
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Inter- and intracellular signal systems
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Motlík, Jan Kubelka, Michal Hajduch, M. Wolf, E. Alberio, R. Zakhartchenko, V.
http://linked.open...n/vavai/riv/zamer	Základní výzkum genetických struktur, fyziologických funkcí a vývojové biologie obratlovců se zaměřením na biomedicínsky, hospodářsky i jinak významné druhy
issn	1040-452X
number of pages	11 (xsd:int)

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