About: Electrochemical surface plasmon resonance biosensor for study of DNA desorption and hybridization

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We report a system, which combines electrochemical and surface plasmon resonance (SPR) techniques on the same sensing chip. Each channel of a four-channel laboratory SPR sensor is supplemented with two planar gold electrodes (the reference and the counter electrodes), whereas the gold layer of SPR chip is used as the working electrode. A custom electronics enables to set an arbitrary potential between the reference and working electrodes and to measure the current flow between the counter and the working electrodes. Information from standard electrochemical techniques, i.e. cyclovoltammetry and chronoamperometry can be acquired with the system while simultaneously monitoring the shift in the surface plasmon resonance. The electrochemical SPR biosensor was used to study desorption of thiolated DNA probes with a negative potential. By comparing the acquired electrochemical and SPR signals, we show that DNA probes as well as a monolayer of alkanethiols can be desorbed by applying negative potentials to the SPR chip surface. Moreover, it is shown that the DNA probes can be reabsorbed on the SPR sensor surface and the complementary DNA can be detected without loss in detection sensitivity. We report a system, which combines electrochemical and surface plasmon resonance (SPR) techniques on the same sensing chip. Each channel of a four-channel laboratory SPR sensor is supplemented with two planar gold electrodes (the reference and the counter electrodes), whereas the gold layer of SPR chip is used as the working electrode. A custom electronics enables to set an arbitrary potential between the reference and working electrodes and to measure the current flow between the counter and the working electrodes. Information from standard electrochemical techniques, i.e. cyclovoltammetry and chronoamperometry can be acquired with the system while simultaneously monitoring the shift in the surface plasmon resonance. The electrochemical SPR biosensor was used to study desorption of thiolated DNA probes with a negative potential. By comparing the acquired electrochemical and SPR signals, we show that DNA probes as well as a monolayer of alkanethiols can be desorbed by applying negative potentials to the SPR chip surface. Moreover, it is shown that the DNA probes can be reabsorbed on the SPR sensor surface and the complementary DNA can be detected without loss in detection sensitivity. (en)
Title	Electrochemical surface plasmon resonance biosensor for study of DNA desorption and hybridization Electrochemical surface plasmon resonance biosensor for study of DNA desorption and hybridization (en)
skos:prefLabel	Electrochemical surface plasmon resonance biosensor for study of DNA desorption and hybridization Electrochemical surface plasmon resonance biosensor for study of DNA desorption and hybridization (en)
skos:notation	RIV/67985882:_____/13:00396553!RIV14-MSM-67985882
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(LH11102)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Chadt, Karel Tichý, Ivo Homola, Jiří Šípová, Hana
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fotoniky a elektroniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	72368
http://linked.open...ai/riv/idVysledku	RIV/67985882:_____/13:00396553
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	regeneration; desorption; SPR sensors (en)
http://linked.open.../riv/klicoveSlovo	SPR sensors desorption regeneration
http://linked.open...ontrolniKodProRIV	[7ECB9ABE9606]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	BELLINGHAM
http://linked.open...i/riv/nazevZdroje	OPTICAL SENSORS 2013
http://linked.open...in/vavai/riv/obor	JA
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Surface Plasmon Resonance Based Biosensing Using Hybrid Nanoplasmonic Materials Fabricated by Self-Assembly Processes
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Homola, Jiří Šípová, Hana Chadt, Karel Tichý, Ivo Ferrari, L.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000323326200012
http://linked.open.../riv/zahajeniAkce	2013-04-15 (xsd:date)
issn	0277-786X
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1117/12.2018462
http://purl.org/ne...btex#hasPublisher	SPIE
https://schema.org/isbn	978-0-8194-9576-1

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