About: Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma

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About: Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We report on the use of new biofunctionalized gold nanoparticles (bio-AuNPs) that enable a surface plasmon resonance (SPR) biosensor to detect low levels of carcinoembryonic antigen (CEA) in human blood plasma. Bio-AuNPs consist of gold nanoparticles functionalized both with (1) streptavidin, to provide high affinity for the biotinylated secondary antibody used in the second step of the CEA sandwich assay, and with (2) bovine serum albumin, to minimize the nonspecific interaction of the bio-AuNPs with complex samples (blood plasma). We demonstrate that this approach makes it possible for the SPR biosensor to detect CEA in blood plasma at concentrations as low as 0.1 ng/mL, well below normal physiological levels (approximately nanograms per milliliter). Moreover, the limit of detection achieved using this approach is better by a factor of more than 1,000 than limits of detection reported so far for CEA in blood plasma using SPR biosensors. We report on the use of new biofunctionalized gold nanoparticles (bio-AuNPs) that enable a surface plasmon resonance (SPR) biosensor to detect low levels of carcinoembryonic antigen (CEA) in human blood plasma. Bio-AuNPs consist of gold nanoparticles functionalized both with (1) streptavidin, to provide high affinity for the biotinylated secondary antibody used in the second step of the CEA sandwich assay, and with (2) bovine serum albumin, to minimize the nonspecific interaction of the bio-AuNPs with complex samples (blood plasma). We demonstrate that this approach makes it possible for the SPR biosensor to detect CEA in blood plasma at concentrations as low as 0.1 ng/mL, well below normal physiological levels (approximately nanograms per milliliter). Moreover, the limit of detection achieved using this approach is better by a factor of more than 1,000 than limits of detection reported so far for CEA in blood plasma using SPR biosensors. (en)
Title	Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma (en)
skos:prefLabel	Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma (en)
skos:notation	RIV/67985882:_____/12:00396508!RIV14-MSM-67985882
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GBP205/12/G118), P(LH11102), P(OC09058)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Homola, Jiří Špringer, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fotoniky a elektroniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	125044
http://linked.open...ai/riv/idVysledku	RIV/67985882:_____/12:00396508
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Surface plasmon resonance; Carcinoembryonic antigen; Biosensor (en)
http://linked.open.../riv/klicoveSlovo	Surface plasmon resonance Carcinoembryonic antigen Biosensor
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[F70E50B0C91C]
http://linked.open...i/riv/nazevZdroje	Analytical and Bioanalytical Chemistry
http://linked.open...in/vavai/riv/obor	JB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Plasmonic nanostructures and functionalizations for optical affinity biosensors Nanobiophotonics for future health care Surface Plasmon Resonance Based Biosensing Using Hybrid Nanoplasmonic Materials Fabricated by Self-Assembly Processes
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Homola, Jiří Špringer, Tomáš
http://linked.open...ain/vavai/riv/wos	000311310100010
issn	1618-2642
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00216-012-6308-9

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