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  • Optical fiber biosensors based on monitoring of cell bioluminescence are employed as environmental pollution sensors. An active layer containing bioluminescent cells on the fiber element end face is a basal part of an optical fiber biosensor (OFB). The number of immobilized living cells and light coupling efficiency determine of OFB sensitivity. In this paper, two shapes of optical fiber elements (OFE) are compared; 600 μm polymer cladded silica (PCS) fiber, and tapered quartz element with 6.3 mm diameter on the front end narrowing to 800 μm (length 32 cm). On these ends, cells Pseudomonas putida TV A8 were immobilized in silica matrix. Bioluminescence measurements confirmed a proportionality between the number of cells and bioluminescence. The number of immobilized cells was 105 times higher on the tapered OFE end as compared to the 600 μm PCS fiber. The measured bioluminescence intensity increased 4 times in the tapered OFE case.
  • Optical fiber biosensors based on monitoring of cell bioluminescence are employed as environmental pollution sensors. An active layer containing bioluminescent cells on the fiber element end face is a basal part of an optical fiber biosensor (OFB). The number of immobilized living cells and light coupling efficiency determine of OFB sensitivity. In this paper, two shapes of optical fiber elements (OFE) are compared; 600 μm polymer cladded silica (PCS) fiber, and tapered quartz element with 6.3 mm diameter on the front end narrowing to 800 μm (length 32 cm). On these ends, cells Pseudomonas putida TV A8 were immobilized in silica matrix. Bioluminescence measurements confirmed a proportionality between the number of cells and bioluminescence. The number of immobilized cells was 105 times higher on the tapered OFE end as compared to the 600 μm PCS fiber. The measured bioluminescence intensity increased 4 times in the tapered OFE case. (en)
Title
  • Optical Fiber Elements for Increasing Bioluminescence Coupling
  • Optical Fiber Elements for Increasing Bioluminescence Coupling (en)
skos:prefLabel
  • Optical Fiber Elements for Increasing Bioluminescence Coupling
  • Optical Fiber Elements for Increasing Bioluminescence Coupling (en)
skos:notation
  • RIV/67985882:_____/09:00375644!RIV12-MSM-67985882
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ME 892), P(ME 893), P(MEB090817), P(OC 121), Z(AV0Z20670512), Z(AV0Z40720504)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 331812
http://linked.open...ai/riv/idVysledku
  • RIV/67985882:_____/09:00375644
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • fiber taper; pseudomonas putida TVA8; bioluminiscence (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [479214645455]
http://linked.open...v/mistoKonaniAkce
  • Bratislava
http://linked.open...i/riv/mistoVydani
  • Bratislava
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  • Proceedings
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http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Kuncová, Gabriela
  • Pospíšilová, Marie
  • Vrbová, H.
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Slovenská technická univerzita v Bratislave
https://schema.org/isbn
  • 978-80-227-3105-8
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