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  • Sensitive coatings are described for an optical sensor for in-situ monitoring of reactants, such as glucose, in biotechnological processes. Glucose oxidase, incorporated into coating materials that are applied on lenses or optical fibers, is used to catalyze oxidization of glucose to gluconic acid. The presence and consumption of oxygen is determined by measuring the fluorescence signal of incorporated ruthenium complexes, which is quenched by oxygen. Inorganic–organic polymers, synthesized via sol-gel processing, were used as coating material. Due to the hybrid character of the coating, good adhesion is achieved on both glass and polymer surfaces. The sensitive optical coating was built up as double-layer and single-layer structures. The double layer comprised a primary coating containing the oxygen-sensitive ruthenium complex, and a secondary coating containing the enzyme. The single layer comprised a single coating containing both the ruthenium complex and the enzyme.
  • Sensitive coatings are described for an optical sensor for in-situ monitoring of reactants, such as glucose, in biotechnological processes. Glucose oxidase, incorporated into coating materials that are applied on lenses or optical fibers, is used to catalyze oxidization of glucose to gluconic acid. The presence and consumption of oxygen is determined by measuring the fluorescence signal of incorporated ruthenium complexes, which is quenched by oxygen. Inorganic–organic polymers, synthesized via sol-gel processing, were used as coating material. Due to the hybrid character of the coating, good adhesion is achieved on both glass and polymer surfaces. The sensitive optical coating was built up as double-layer and single-layer structures. The double layer comprised a primary coating containing the oxygen-sensitive ruthenium complex, and a secondary coating containing the enzyme. The single layer comprised a single coating containing both the ruthenium complex and the enzyme. (en)
Title
  • Hybrid Coatings as Transducer in Optical Biosensors
  • Hybrid Coatings as Transducer in Optical Biosensors (en)
skos:prefLabel
  • Hybrid Coatings as Transducer in Optical Biosensors
  • Hybrid Coatings as Transducer in Optical Biosensors (en)
skos:notation
  • RIV/67985858:_____/08:00388346!RIV13-AV0-67985858
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z20670512), Z(AV0Z40720504)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 370939
http://linked.open...ai/riv/idVysledku
  • RIV/67985858:_____/08:00388346
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • optical sensor; fluorescence; hybrid coating (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [7574AA0E4C44]
http://linked.open...i/riv/nazevZdroje
  • Journal of Coatings Technology and Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 5
http://linked.open...iv/tvurceVysledku
  • Kuncová, Gabriela
  • Matějec, Vlastimil
  • Rose, K.
  • Dzyadevych, S.
  • Fernandez-Lafuente, R.
  • Jaffrezic, N.
  • Scully, P.
http://linked.open...ain/vavai/riv/wos
  • 000260512100010
http://linked.open...n/vavai/riv/zamer
issn
  • 1547-0091
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s11998-008-9082-z
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