About: Monitoring of the Viability of Cells Immobilized by Sol-Gel Process

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Three different types of cells, Pseudomonas fluorescens HK44, Saccharomyces cerevisiae strain SP4 and plant cells Nicotiana tabacum L. BY-2, were immobilized by entrapment in tetramethoxysilane prepolymer (TMOS) gel or in composite gel containing prepolymer TMOS and alginate in various ratios. Their growth and viability were monitored by bioluminescence and 2-D fluorescence spectra, which are fast and do not need the dissolution of a matrix. The resulting biocomposite gels were obtained by gelation of the mixtures of TMOS prep. or TMOS/alginate sols and the particular cells in proper media on glass supports to provide films ~1 mm thick. The effect of the following parameters on the growth and viability of the cells was studied: (a) the composition of the biocomposites, (b) the preparation conditions of TMOS and (c) the conditions of the procedure of entrapment. All three types of cells were tested in TMOS gel and the composite TMOS/alginate = 1:1 (v/v). Three different types of cells, Pseudomonas fluorescens HK44, Saccharomyces cerevisiae strain SP4 and plant cells Nicotiana tabacum L. BY-2, were immobilized by entrapment in tetramethoxysilane prepolymer (TMOS) gel or in composite gel containing prepolymer TMOS and alginate in various ratios. Their growth and viability were monitored by bioluminescence and 2-D fluorescence spectra, which are fast and do not need the dissolution of a matrix. The resulting biocomposite gels were obtained by gelation of the mixtures of TMOS prep. or TMOS/alginate sols and the particular cells in proper media on glass supports to provide films ~1 mm thick. The effect of the following parameters on the growth and viability of the cells was studied: (a) the composition of the biocomposites, (b) the preparation conditions of TMOS and (c) the conditions of the procedure of entrapment. All three types of cells were tested in TMOS gel and the composite TMOS/alginate = 1:1 (v/v). (en) Tři různé typy buněk, Pseudomonas fluorescens HK44, Saccharomyces cerevisiae strain SP4 and plant cells Nicotiana tabacum L. BY-2, byly imbilizovány zachycením v předpolymerizovaném tetramethoxysilanu a kompositním gelu složeném z předpolymerovaného teramethoxysilanu a alginatu. Viabilita bakterii byla ve srovnáním s kvasinkami a rostlinými buňkami snížna v důsledku imobilizace nejméně. (cs)
Title	Monitoring of the Viability of Cells Immobilized by Sol-Gel Process Sledování viability buněk imobilizovaných procesem sol-gel (cs) Monitoring of the Viability of Cells Immobilized by Sol-Gel Process (en)
skos:prefLabel	Monitoring of the Viability of Cells Immobilized by Sol-Gel Process Sledování viability buněk imobilizovaných procesem sol-gel (cs) Monitoring of the Viability of Cells Immobilized by Sol-Gel Process (en)
skos:notation	RIV/67985858:_____/04:00105015!RIV/2005/MSM/A27005/N
http://linked.open.../vavai/riv/strany	335;342
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA104/01/0461), P(OC 840.10), P(OC 840.20), Z(AV0Z4072921)
http://linked.open...iv/cisloPeriodika	1-3
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Kuncová, Gabriela Podrazký, Ondřej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav chemických procesů AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	574480
http://linked.open...ai/riv/idVysledku	RIV/67985858:_____/04:00105015
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	sol-gel process;cell entrapment;viability (en)
http://linked.open.../riv/klicoveSlovo	viability sol-gel process cell entrapment
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[633B5199E353]
http://linked.open...i/riv/nazevZdroje	Journal of Sol-Gel Science and Technology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Permeable barriers with immobilized bacteria treating mixed pollutans in the environment http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20840.20 http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20840.10
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Kuncová, Gabriela Podrazký, Ondřej Trögl, Josef Vaňková, Radomíra Demnerová, K. Ripp, S. Sayler, G. S.
http://linked.open...n/vavai/riv/zamer	Theoretical fundamentals of chemical processes: equilibrium and dynamic behavior of multiphase reacting systems
issn	0928-0707
number of pages	8 (xsd:int)

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