About: Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation

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About: Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In order to control the hydrophobic or hydrophilic character, the NCD film surfaces were grafted by hydrogen atoms (H-termination) or by oxygen atoms (O-termination). Human mesenchymal stem cells and primary human osteoblasts were used for biological studies on H- and O-terminated NCD films. We found that cells cultivated on O-terminated NCD films exhibit better adhesion than H-terminated NCD films. In addition, the expression of osteogenic cell markers collagen and alkaline phosphatase analysed by Realtime PCR and immunostaining was higher on O-terminated films. In order to control the hydrophobic or hydrophilic character, the NCD film surfaces were grafted by hydrogen atoms (H-termination) or by oxygen atoms (O-termination). Human mesenchymal stem cells and primary human osteoblasts were used for biological studies on H- and O-terminated NCD films. We found that cells cultivated on O-terminated NCD films exhibit better adhesion than H-terminated NCD films. In addition, the expression of osteogenic cell markers collagen and alkaline phosphatase analysed by Realtime PCR and immunostaining was higher on O-terminated films. (en)
Title	Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation (en)
skos:prefLabel	Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation (en)
skos:notation	RIV/67985823:_____/14:00440278!RIV15-GA0-67985823
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0109), P(EE2.3.30.0025), P(GA14-04790S)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Lišková, Jana Bačáková, Lucie Švindrych, Zdeněk Hadraba, Daniel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	13308
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/14:00440278
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nanocrystalline diamond film; osteoblast; MSC; type I collagen; paxillin (en)
http://linked.open.../riv/klicoveSlovo	nanocrystalline diamond film type I collagen osteoblast MSC paxillin
http://linked.open...ontrolniKodProRIV	[90486525129B]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Ostrava
http://linked.open...i/riv/nazevZdroje	NANOCON 2014 Conference Proceedings
http://linked.open...in/vavai/riv/obor	BM
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University Center of Biomedical Research Engineering Bulk and Surface of Diamond Nano-Objects for Biomedicine
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Babchenko, Oleg Bačáková, Lucie Kromka, Alexander Lišková, Jana Švindrych, Zdeněk Varga, Marián Hadraba, Daniel
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2014-11-05 (xsd:date)
number of pages	7 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Tanger s.r.o.
https://schema.org/isbn	978-80-87294-55-0

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